鸡传染性法氏囊病病毒与DT40 sIgM λ轻链的相互作用分析  

Analysis of the Interactions between sIgM λ Light Chain of DT40 Cells and Infectious Bursal Disease Virus

作　　者：罗俊[1] 滕蔓[1] 邓瑞广[1] 杨继飞[1] 赵东[1] 柴书军[1] 叶世同 孙玉梅 张改平[1] 

机构地区：[1]河南省农业科学院农业部动物免疫学重点开放实验室河南省动物免疫学重点实验室,河南郑州450002 [2]罗山县动物卫生监督所,河南罗山464237

出　　处：《河南农业科学》2009年第9期177-180,183,共5页Journal of Henan Agricultural Sciences

基　　金：国家科技支撑计划项目(2006BAD06A04-6);国家"973"计划项目(2005CB522800);河南省基础与前沿计划项目(082300433201)

摘　　要：为探讨sIgM λ轻链在IBDV感染法氏囊B淋巴靶细胞过程中的作用,对DT40细胞sIgM λ轻链与IBDV的相互作用进行了研究。利用蛋白表达、VOPBA试验、病毒结合与结合抑制试验检测了sIgM λ轻链及DT40细胞结合IBDV的能力。结果表明:sIgM λ轻链在体外能够特异性地结合多种不同毒株的IBDV,这种结合与病毒毒力无关;病毒结合与结合抑制试验结果表明,超过半数的DT40细胞可以结合IBDV,这种结合能够被sIgM λ轻链特异性单抗有效阻断。研究结果表明,sIgM λ轻链是DT40细胞膜表面上IBDV的重要结合位点之一,这为进一步利用DT40细胞研究IBDV感染B淋巴靶细胞的分子机制提供了重要线索。To investigate the role of sIgM playing in the infection process of infectious bursal disease virus （IBDV）, the interactions between sIgM λ light chain and IBDV was systematically studied by using DT40, a sIg M-bearing cell line derived from chicken busal lymphoma. The experiments of protein expression, VOPBA, virus binding and binding inhibition were performed to evaluate the binding abilities of DT40 cells and sIgM λ light chain to IBDV particles. VOPBA showed that the ）, light chain of slgM specifically interacted with 4 distinct strains of IBDV in a virulence independent manner. Flow cytometric analysis also showed that more than half of DT40 cells bound IBDV particles, and the binding was efficiently inhibited by sIgM -specific monoclonal antibody （mAb） L1. Our data indicates that sIgM λ light chain plays as an important site for the binding of IBDV, which provided some important clues for the further investigations of the molecular pathogenesis of IBDV infection to B lymphocytes using DT40 as the target cell model.

关 键 词：传染性法氏囊病病毒 DT40细胞 sIgMλ轻链 分子致病机制 

分 类 号：S858.31[农业科学—临床兽医学]