纳米TiO_2对胰蛋白酶的物理吸附作用研究  

Research on Physical Absorption Behavior of Trypsin on Nano-meter TiO_2

作　　者：彭红[1] 章晓[1] 范良松[1] 黄开勋[1] 

机构地区：[1]华中科技大学化学与化工学院,武汉430074

出　　处：《武汉理工大学学报》2009年第17期88-91,共4页Journal of Wuhan University of Technology

基　　金：国家"973"计划(2006CB705606A)

摘　　要：不同合成方法得到的纳米TiO2的粒径大小、形貌、晶型及等电点等表面特性对物理吸附胰蛋白酶有较大的影响。分别应用均匀沉淀法、溶胶-凝胶法和水热法合成了3种TiO2纳米粒。XRD分析表明3种产物的晶型分别为锐钛矿型、金红石型和金红石型。TEM分析表明3种产物的粒径分别为小于5 nm、10—100 nm和30 nm。此外3种产物的等电点分别为3.0、6.1和5.2。分别应用这3种纳米粒物理吸附胰蛋白酶,结果表明在pH 5.0—6.0时,均匀沉淀法产物对胰蛋白酶的偶联效率及吸附蛋白质的能力最高,其次为水热法产物。溶胶-凝胶法产物粒径分布不均匀,且100 nm左右的大颗粒较多,因此它的偶联效率及吸附蛋白质的能力最差。由此可见纳米TiO2对胰蛋白酶的物理吸附作用与溶液的pH及粒径大小密切相关。当蛋白质溶液的pH为5.0—6.0时,TiO2粒径越小,对胰蛋白酶的吸附能力越强。The structures and surface properties such as particle sizes, crystals and morphology of nano-TiO2 prepared by different methods have great effects on physical absorption of trypsin. In this paper, three kinds of TiO2 nanoparticles are synthesized by hydrothermal method, sol-gel method and homogeneous precipitation method respectively. The crystal structures of these TiO2 nanoparticles are rutile, rutile and anatase. Their particle sizes are about 30 nm, 10-100 nm and smaller than 5 nm measured by TEM respectively. Their isoelectric points are about 5.2, 6.1 and 3.0 respectively. These TiO2 nanoparticles are used to immobilize trypsin by physical absorption respectively. The results show that the trypsin coupling efficient and abilities of absorption of proteins on TiO2 prepared by homogeneous precipitation method are the best during pH 5.0 to pH 6.0, and that on TiO2 prepared by hydrothermal method is in the middle, but that on TiO2 prepared by sol-gel method is the worst because of its irregular shapes and lots of particle sizes above 100 nm. In conclusion, the pH value of solution and the particle sizes of titanic oxide have great effects on physical absorption of trypsin on TiO2. The smaller the particles sizes of titanic oxide, the better the abilities of absorption of trypsin in the protein solution during pH 5.0 to pH 6.0.

关 键 词：TIO2 纳米粒 物理吸附 胰蛋白酶 

分 类 号：O69[理学—化学]