反相高效液相色谱法测定犬血浆吗啡浓度  

Determination of Morphine Concentrations in Dog Plasma by RP-HLPC

作　　者：袁盛华[1] 潘琳[2] 谢杰[3] 徐鑫[4] 汪晓海[4] 葛卫红[1] 

机构地区：[1]南京大学医学院附属鼓楼医院药剂科,210008 [2]南京大学医学院,210008 [3]江苏省徐州医学院临床药学系,221004 [4]南京大学医学院附属鼓楼医院麻醉科,210008

出　　处：《医药导报》2009年第9期1107-1109,共3页Herald of Medicine

基　　金：南京市卫生局医学科技发展一般课题(基金编号:YKK05106)

摘　　要：目的建立犬血浆吗啡浓度反相高效液相色谱测定方法。方法犬血浆样品中加入内标对乙酰氨基酚后用乙酸乙酯在碱性条件下提取。流动相为0.2%三乙胺水溶液(pH为6.89)-甲醇(78:22);Kromasil C18柱(150 mm×4.6 mm,5μm);流速1 mL.min-1;紫外检测波长215 nm。结果吗啡线性范围为5～2 000 ng.mL-1(r=0.999 4);最低检出浓度2 ng.mL-1;提取回收率74.92%～89.34%,方法回收率84.29%～106.45%;日内和日间RSD分别为3.71%～9.31%和5.14%～12.38%。结论该方法处理简单、灵敏、准确,可用于犬血浆吗啡浓度测定。Objective To develop an RP-HPLC method for determining concentrations of morphine in clog plasma. Methods With paracetamoi as internal standard, morphine in clog plasma was extracted with ethyl acetate and then determined by RP-HPLC with the conditions as follows： Kromasil Cls column （150 mm × 4.6 mm, 5 μm） , a mobile phase of 0.2% triethylamine solution（ pH 6.89）-methanol （78 ： 22, V/V） with the velocity of 1 mL ·main-1 and the absorption wave length was 215 nm. Results The sample has been quantified over a concentration range of 5 to 2 000 ng · mL-1 （ r = 0. 999 4） ; the minimal detectable concentration was 2 ng · mL-1 , the extraction recovery was 74.92% - 89.34%, and the method recovery was 84.29% - 106.45%. Intra-day and inter-clay RSD were 3.71% -9.31% and 5.14% - 12.38%, respectively. Conclusion This method is simple, sensitive and accurate for determining morphine concentrations in dog plasma.

关 键 词：吗啡 色谱法 高效液相 反相 犬 

分 类 号：R971.2[医药卫生—药品] R969.1[医药卫生—药学]