冬凌草甲素对MCF-7细胞的生长抑制作用及诱导凋亡作用的实验研究  

Growth Inhibitory Effect and Inducing Apoptosis Effect of Oridonin on Human Breast Cancer MCF-7 Cells

作　　者：朱仁书[1] 李希科[1] 

机构地区：[1]鹤壁职业技术学院护理系河南,鹤壁市458030

出　　处：《医药论坛杂志》2009年第16期3-5,共3页Journal of Medical Forum

摘　　要：目的探讨冬凌草甲素对人乳腺癌细胞MCF-7细胞的生长抑制作用及诱导凋亡作用。方法以不同浓度的冬凌草甲素作用于体外培养的MCF-7细胞,MTT法检测细胞生长抑制率,应用Hoechst 33258荧光染色法以及流式细胞仪观察细胞凋亡情况。结果冬凌草甲素可显著抑制MCF-7细胞的生长及诱导细胞发生凋亡,并呈现出一定的剂量-效应与时间-效应关系。Hoechst 33258荧光染色观察到典型的核浓缩、核碎裂等细胞凋亡的形态学变化。结论冬凌草甲素能显著抑制MCF-7细胞的生长,诱导细胞发生凋亡是其重要作用方式。Objective To investigate the growth inhibitory effect and inducing apoptosis effect of oridonin on MCF -7 cells. Methods Differe.nt concentrations of oridonin were added in culture medium. The growth inhibitory rate of the MCF -7 cells was measured by MTT assay, cell apoptotic rate was detected by flow cytometry （ FCM ）, morphology of apoptosis was observed by Hoechst 33258 fluorescence staining. Results Oridonin could inhibit the growth of MCF -7 cells and induce cell apoptosis significantly. The suppression was both in a time - and dose - dependent manner. Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining. Conclusion Oridonin had growth inhibitory effect on MCF- 7 cells in vitro,which associated with the cell apoptosis.

关 键 词：冬凌草甲素 MCF-7细胞 生长抑制 细胞凋亡 

分 类 号：R979.1[医药卫生—药品]