L-Arg-NO途径对铝抑制大鼠海马CA3区诱发电位的影响  

Influence of L-Arginine-NO Pathway on the Evoked Potential Inhibited by Aluminium in Hippocampal CA3 Area of Rats

作　　者：肖鸿美[1] 邹本德[1] 张自东[1] 李艾 

机构地区：[1]同济医科大学基础医学院生理学教研室,武汉430030

出　　处：《同济医科大学学报》1998年第2期100-104,共5页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金资助项目(No.39270591)

摘　　要：实验在70只乌拉坦麻醉的SD大鼠上进行。在海马CA3区记录诱发的群体锋电位（PS）,探讨了左旋精氨酸——氧化氮（L-Arg-NO）途径对铝抑制PS波幅的作用。结果:（1）0.5 mol/L AICI_3注入CA3区后,PS幅度较注药前显著减小（P<0.05）。（2）CA3区内注入0.1和 0.3mol/L硝基左旋精氨酸（ NLA）能分别加强0.25和0.5 mol/L AICl_3 的抑制效应。（3）CA3区注入0.3 mol/L左旋精氨酸（L-Arg）能拮抗0.5 mol/L AICI_3 对PS波幅的抑制作用,此拮抗作用并能为 NLA所翻转。（4）CA3区内注入 0.2mmol/L亚甲蓝（MB）能加强铝对PS的抑制作用。结果提示:AICI_3对CA3区诱发的PS幅度有抑制作用,此作用可能与L-Arg-NO途径受到损害有关。Experiments were performed on 70 Sprague-Dawley rats under urethane anesthesia. Extracellular recording method was used to investigate the effect of aluminium (AD microinjected into CA3 on evoked potential in hippocampal CA3 area and the relationship between Al and L-Arg-NO pathway in the inhibitory effect of aluminium. The results were as follows, (1) Aluminium (0. 5mol/L,1 μl) microinjected into CA3 could decrease the amplitude of PS in CA3 significantly(P<0.05). (2) Nitro-L-Arginine (NLA, 0. 1 mol/L, 0. 3 mol/L) microinjected into CA3 could enhance the inhibitory effect of Al (0. 25 mol/L,0. 5 mol/L) on PS recorded in CA3 (P<0. 01 or P<0. 05). (3) The inhibitory effect of 0. 5 mol/L Al microinjected into CA3 could be antagonised by preinjection of L-arginine (L-Arg) (P<0. 01; P<0. 05), the effect of L-Arg could be reversed by 0. 3 mol/L NLA. (4) The inhibitory effect of Al on PS could be enhanced by microinjection of 0. 2 mmol/L methylene blue(MB) in CA3 (P<0.01). These results suggest that Al can inhibit the PS induced in CA3 and that this effect of Al might be related to impairment of L-Arg-NO pathway.

关 键 词：左旋精氨酸 硝基 亚甲蓝 海马 CA3区 诱发电位 

分 类 号：Q517[生物学—生物化学] R322.81[医药卫生—人体解剖和组织胚胎学]