IL-1β和IL-2对培养的大脑皮质神经元Glu和GABA免疫反应的影响  

The Effects of Interleukin-1β and Interleukin-2 on Glu and GABA Immunoreaction in Cultured Neurons of Rat Cerebral Cortex

作　　者：李正莉[1] 朱长庚[1] 周洁萍[1] 魏瑛[1] 刘云华[1] 

机构地区：[1]同济医科大学基础医学院解剖学教研室

出　　处：《同济医科大学学报》1998年第2期105-107,170,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金资助重点课题(No.39330210)

摘　　要：为了研究细胞因子白细胞介素－１（ＩＬ－１）和白细胞介素－２（ＩＬ－２）对神经系统兴奋性的调节作用，将ＩＬ－１β和ＩＬ－２分别作用于培养的大鼠大脑皮质神经元，然后观察其对谷氨酸（Ｇｌｕ）和ｒ－氨基丁酸（ＧＡＢＡ）免疫反应神经元的影响，并对实验结果进行了图像分析和统计学处理，结果显示：ＩＬ－１β（１０＾５ｕ／Ｌ）作用于培养的神经元后，Ｇｌｕ免疫反应神经元增加（Ｐ〈０．０１）。The study was aimed to investigate the regulating roles of IL-1β and IL-2 on the excitement of nervous system. Their effects on Glu and GABA immunoreaction of cultured neurons of cerebral cortex of the rat were observed . The results were analysed by image analysis and statistics and showed that Glu im-munoreactive neurons were significantly increased (P<0. 01), but no change was found in GABA immunore-active neurons following the treatment of IL-1β(105 u/L); whereas Glu immunoreactive neurons were increased (P<0. 05) and GABA immunoreactive neurons were greatly reduced (P<0. 01) following the treatment of IL-2(104 u/L). The above results suggest that both IL-1B and IL-2 may enhance the excitement of neurons of cerebral cortex by regulating the metabolism of Glu and GABA in the brain. The results also provide morphology information for the Immuno-Neuro-Endocrine network.

关 键 词：IL-1Β IL-2 谷氨酸 氨基丁酸 神经元 

分 类 号：R392.12[医药卫生—免疫学] R322.8[医药卫生—基础医学]