NF-кB抑制剂对大鼠AMI再灌注后无再流及ICAM-1,P-选择素表达的影响  

Effect of NF-кB inhibitor on no-reflow and expression of ICAM-1 and P-selectin

作　　者：何泉[1] 雷寒[1] 柳青[1] 覃数[1] 马康华[1] 王曦[1] 

机构地区：[1]重庆医科大学附属第一医院心内科,重庆400016

出　　处：《第四军医大学学报》2009年第17期1545-1548,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30670869)

摘　　要：目的:探讨核因子кB抑制剂二硫代氨基甲酸吡咯烷(PDTC)对大鼠急性心肌梗死(AMI)再灌注后无再流及细胞间粘附分子-1(ICAM-1),P-选择素表达调控的影响.方法:将36只成年SD大鼠随机分为假手术组、对照组及PDTC组.结扎冠脉左前降支3h继而松解2h建立无再流动物模型.再灌注后2h采用病理染色法检测无再流范围;采用免疫荧光组化、RT-PCR方法检测ICAM-1,P-选择素蛋白及mRNA的表达.结果:对照组缺血区ICAM-1,P-选择素蛋白及mRNA表达较假手术组显著升高(P<0.01),而PDTC使缺血区ICAM-1,P-选择素蛋白及mRNA表达较对照组显著减弱(P<0.05);PDTC组无再流范围较对照组显著减小(P<0.05).结论:NF-кB抑制剂PDTC能显著减小大鼠AMI再灌注后无再流范围,其作用可能与抑制再灌注后缺血区ICAM-1及P-选择素的表达有关.AIM ： To investigate the effect of pyrrolidine dithiocarbamate （ PDTC ） , an inhibitor of nuclear faetor-KB （ NF-κB ） , on the no reflow after reperfusion and the expressions of intercellular adhesion molecule-1 （ICAM-1）and P-selectin in rats with acute myocardial infarction （ AMI ）. METHODS ： Thirty-six SD rats were randomly divided into sham-operated group, control group and PDTC group. To establish the animal model of no reflow, the left anterior descending coronary arteries of rats underwent 3 h of ligation and 2 h of reperfusion. The area of no-reflow （ ANR% ） was determined by pathological staining and proteins and mRNA expressions of ICAM-1 and P-selectin were detected by immuno-fluorescent staining and RT-PCR. RESULTS： Compared with those in sham-operated group, the proteins and mRNA expression of ICAM-1 and P-selectin in the ischemic area significantly increased in control group（ P 〈 0.01 ）, while those in PDTC group were significantly inhibited（ P 〈 0.05 ）. The area of no reflow in PDTC group significantly decreased compared with that in control group（ P 〈 0.05 ）. CONCLUSION ： PDTC significantly reduces the area of no-reflow after reperfusion in rats with AMI, possibly by the suppression of ICAM-1 and P-selectin gene expressions.

关 键 词：急性心肌梗死 无再流现象 P-选择素 细胞间粘附分子-1 核因子-κB 

分 类 号：R542.22[医药卫生—心血管疾病] R73-3[医药卫生—内科学]