不同种属来源真皮间充质干细胞对人皮肤成纤维细胞组织修复作用的影响  

Effect of heterogeneously dermis-derived mesenchymal stem cells on human skin fibroblasts tissue repair

作　　者：毛越苹[1] 齐庆[2] 谭国珍[1] 郭庆[1] 曾凡钦[1] 

机构地区：[1]中山大学附属第二医院皮肤性病科,广东广州510120 [2]广州中医药大学附属第一医院皮肤性病科,广东广州510405

出　　处：《第四军医大学学报》2009年第17期1587-1590,共4页Journal of the Fourth Military Medical University

摘　　要：目的:比较小鼠真皮间充质干细胞(mdMSCs)和人胚胎真皮间充质干细胞(hdMSCs)对人皮肤成纤维细胞(hsFb)组织修复作用的影响,探讨异种属真皮间充质干细胞(MSCs)促进hsFb组织修复的可靠性.方法:采用低血清培养基,以消化—贴壁—传代法体外培养、鉴定mdMSCs和hdM-SCs,并与体外分离培养的hsFb于transwell培养体系中共培养,采用样本碱水解法和ELISA法分别检测第1,4日培养上清液中羟脯氨酸(Hyp)和转化生长因子-β1(TGF-β1)的变化.结果:不同种属MSCs(mdMSCs/hdMSCs)对共培养上清液Hyp含量的影响差异(F=0.174)以及不同处理组(即不同细胞密度MSCs)培养上清液Hyp含量差异(F=1.304)均无显著性.不同时间(即第1和第4日)共培养上清液Hyp含量差异具有统计学意义(F=8.712,P<0.01).不同种属MSCs(mdMSCs/hdMSCs)对上清液TGF-β1的含量影响差异(F=0.062)以及不同处理组(即不同细胞密度MSCs)培养上清液TGF-β1含量差异(F=1.991)均无显著性.不同时间(即第1和第4日)培养上清液TGF-β1含量差异具有统计学意义(F=0.699,P<0.05).结论:不同来源真皮MSCs与hsFb共培养对胶原分泌和TGF-β1表达无影响,组织修复过程中,异种属真皮MSCs的诱导是可靠的.AIM： To compare the tissue repair effect of mouse dermis-derived mesenchymal stem eells（mdMSCs）and human dermis-derived MSCs（hdMSCs） on normal human skin fibroblast （hsFb） and to explore the reliability of heterogeneously dermis-derived MSCs in tissue repair. METHODS： mdMSCs and hdMSCs were isolated and cultivated by tissue digested-adherent-passage in lowserum medium and the changes of hydroxyproline （Hyp） and TGF-β1 were determined in co-culture system of mdMSCs or hdMSCs and hsFb on day 1 and 4 by samples basic hydrolysis and ELISA respectively. RESULTS： No significant difference was found in the contents of Hyp between groups of different MSCs（ F = 0. 174） or between groups of different cell density （ F = 1. 304 ）. The contents of Hyp were significantly different between day 1 and day 4 （ F = 8. 712, P 〈0.01 ）. The level of TGF-β1 was not significantly different between groups of different MSCs（ F = 0.062） or between groups of different cell density（ F = 1. 991 ）. The level of TGF-β1 was significantly different between day 1 and day 4 （ F = 0. 699, P 〈 0. 05 ）. CONCLUSION ： Dermal MSCs of different origin （mouse or human ） would not affect the levels of collagen and TGF-131 when cultivated with hsFb. It is reliable for heterogeneously dermis-derived MSCs to promote tissue repair on hsFb.

关 键 词：真皮 间充质干细胞 异种动物 成纤维细胞 

分 类 号：R593.25[医药卫生—内科学]