rGM-CSF对U937细胞系膜MHC分子及CD86分子表达的作用  

The effects of rGM CSF on MHC and CD86 molecules expression on U937 cells

作　　者：尚红生[1] 吕平[1] 马树俊 尹忆民[1] 

机构地区：[1]北京医科大学

出　　处：《细胞与分子免疫学杂志》1998年第1期8-10,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：本实验研究了人重组ＧＭＣＳＦ对Ｕ９３７人单核细胞样细胞系ＨＬＡ和ＣＤ８６分子表达的调节作用。将Ｕ９３７细胞在ｒＧＭＣＳＦ１０μｇ／Ｌ培养１ｄ，ＨＬＡＤＲ分子的表达率为８３％，对照组为９１％；培养２ｄ，表达率为５１％，对照组为７８％；培养５ｄ，表达率为５３％，对照组为８１％。Ｕ９３７细胞在ｒＧＭＣＳＦ１０μｇ／Ｌ浓度下培养１ｄ，ＣＤ８６分子表达率为２％，对照组为１．６％；培养３ｄ，表达率为３．９１％，对照组为１％；培养５ｄ，表达率为５３２％，对照组为０．５８％。而ＭＨＣⅠ类分子表达率在１０μｇ／Ｌ浓度下培养５ｄ时，实验组和对照组均为１００％。以上结果显示，ｒＧＭＣＳＦ对Ｕ９３７细胞具有下调ＭＨＣⅡ类分子表达和上调ＣＤ８６分子表达的作用。ＭＨＣⅡ类分子和ＣＤ８６分子是Ｔ细胞重要的活化信号分子，因此可以说明，ｒＧＭＣＳＦ对Ｔ细胞参与的免疫应答具有重要的调节意义。We analyzed the effects of human recombinant GM CSF on MHC and CD86 molecule expression on U937 human monocyte like cell line. U937 cells were cultured for one day. The percentages of DR molecule expression were 83% in presence of rGM CSF( 10 μg/L) and medium control was 91%. Cultured for three days, the percentages were 51% and medium control was 78%. Cultured for five days, the percentage was 53% and medium control was 81%. U937 cells were cultured in presence of rGM CSF (10 μg/L) for one day, the percentage of CD86 molecule expression was 2% and medium control was 1.6%. Cultured for three days, the percentages were 3.91% and medium control was 1%. Cultured for five days, the percentage was 5.32% and medium control was 0.58%. The percentage of MHC class Ⅰ molecule wxpression was 100% in presence of rGM CSF (10 μg/L) for five dyas and medium control was also 100%. The data show that rGM CSF dowm regulated DR and up regulated CD86 molecule expression, but did not alter MHC class Ⅰ molecule expression. MHC and CD86 molecules provide a stimulatory signal necessary for T cell activation. These results suggested the role of rGM CSF in immune regulation.

关 键 词：rGM-CSF U937细胞系 MHC分子 CD86 

分 类 号：R392.12[医药卫生—免疫学]