柴胡及甘草酸对小鼠肝细胞凋亡的影响  

Effects of radix bupleuri and glycyrrhizin on the hepatocyte apoptosis in mice

作　　者：陈东鸿[1,2] 李平[1,2] 程建峰[1,2] 仲崇华[1,2] 贺建荣[1,2] 滕树保[1,2] 贺平[1,2] 

机构地区：[1]第四军医大学唐都医院药学部 [2]第二军医大学东方肝胆外科研究所

出　　处：《第四军医大学学报》1998年第3期279-280,共2页Journal of the Fourth Military Medical University

摘　　要：目的：研究柴胡（ＲＢ）及甘草酸（ＧＬ）对小鼠肝细胞凋亡的影响．方法：以肝／体重比、肝组织ＤＮＡ含量、组织学改变及原位凋亡细胞ＴＵＮＥＬ反应为指标，观察ＲＢ（１２．０ｇ／ｋｇ）及ＧＬ（５０ｍｇ／ｋｇ），ｉｐ，１次／８ｈ，对撤除苯巴比妥钠（ＰＢ）后引起的小鼠肝细胞凋亡的影响．结果：与对照组Ｃ相比，ＲＢ组小鼠肝／体重比及肝ＤＮＡ含量无明显回落，组织学检查未见明显凋亡改变，ＴＵＮＥＬ反应阴性．ＧＬ组小鼠肝／体重比及ＤＮＡ含量分别回落１２．０％和１３．６％，肝细胞出现典型凋亡改变，ＴＵＮＥＬ反应阳性．结论：ＲＢ能阻断撤除ＰＢ诱导的小鼠肝细胞凋亡。Aim: The effects of radix bupleuri (RB) and glycyrrhizin(GL)on the hepatocyte apoptosis in mice were investigated. Methods: The ratio of liver/body weight, hepatic DNA content, morphological changes and TUNEL reaction (TdT mediated X dUTP nick labeling), as indices of evaluating hepatocyte apoptosis, were used to observe the effects of RB (12.0 g/kg,ip) and GL (50 mg/kg,ip) on the hepatocyte apoptosis caused by phenobarbital (PB) withdrawal. Results: The regression of hepatic DNA content and ratio of liver/body weight and typical hepatocyte apoptosis, which occurred in the mice as control after stopping PB, were not found in the mice treated with RB,and a negative result of TUNEL assay was presented. However, in the mice given GL the ratio of liver/body weight and hepatic DNA content regressed by 12.0% and 13.6%, respectively; with a positive reaction of TUNEL assay and the typical changes of hepatocyte apoptosis. Conclusion: The results showed that RB, but not GL, prevented hepatocyte apoptosis caused by PB withdrawal in mice.

关 键 词：柴胡 甘草酸 苯巴比妥钠 肝细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]