rIL-2体外激活骨髓细胞的抗肿瘤作用及机理  

Antitumor effect of bone marrow cell activated with rIL 2 in vitro and its mechanism

作　　者：朱步海[1] 杨平地[1] 孙秉中[1] 朱华锋[1] 于文强[1] 

机构地区：[1]第四军医大学西京医院血液内科

出　　处：《第四军医大学学报》1998年第3期283-286,共4页Journal of the Fourth Military Medical University

摘　　要：目的：探讨ｒＩＬ－２体外激活骨髓细胞产生细胞毒活性的作用机制．方法：取人新鲜骨髓中单个核细胞，体外与ｒＩＬ－２孵育２４ｈ及４８ｈ，即激活骨髓（ＡＢＭ），同时设不加ｒＩＬ－２组（ＮＢＭ）为对照．分别检测ＡＢＭ，ＮＢＭ的细胞毒活性、膜表面标志（ｍＴＮＦ－α，ＣＤ１１ｂ）及培养液中ＴＮＦ－α水平，并进行比较观察和相关性分析．结果：ＡＢＭ对ＨＬ－６０细胞的细胞毒活性较ＮＢＭ明显增强，ｍＴＮＦ－α，ＣＤ１１ｂ的表达能力及培养液中ＴＮＦ－α水平明显增高（Ｐ＜０．０５），且ＡＢＭ的ＣＤ１１ｂ表达水平与培养上清中ＴＮＦ－α水平，ＴＮＦ－α水平与同时相的细胞毒活性之间均呈密切正相关（Ｐ＜０．０１）．结论：ｒＩＬ－２激活骨髓细胞分泌释放ＴＮＦ－α参与了ＡＢＭ的杀肿瘤活性，可能与骨髓单核巨噬细胞、中性粒细胞和ＮＫ细胞被激活有关．Aim: The mechanism of the cytotoxic effect of bone marrow cells activated with rIL 2 was observed. Methods: The cytotoxicity of activated bone marrow (ABM), cell surface markers(mTNF α, CD11 b)and the level of TNF α in culture supernatant of ABM were measured separately after bone marrow was activated with rIL 2 for 24 and 48 h. Results: The cytotoxic activity against HL 60 cell line,the expression of CD11 b and the level of TNF α were significantly increased in ABM compared with that in non activated bone marrow (NBM) ( P <0.05). Meanwhile, there were significantly positive relationship between the expression of CD11 b and the level of TNF α in supernatant of ABM, the level of TNF α in suppernant and cytotoxicity of ABM. Conclusion: Bone marrow cells activated with rIL 2 producing TNF α might attend the antitumor effect of ABM ( P <0.01),which probably is associated with the activation of the monocyte macrophage, neutrocyte and NK cell.

关 键 词：RIL-2 白细胞介素2 骨髓细胞 抗肿瘤活性 

分 类 号：R392.12[医药卫生—免疫学]