原核表达的Janusin片段对体外培养脊髓神经细胞形态的影响  

Effects of different fragments of Janusin on neuron morphology in vitro

作　　者：许汉鹏[1] 梁哲[1] 肖华胜[1] 王浩军[1] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所

出　　处：《第四军医大学学报》1998年第3期336-339,共4页Journal of the Fourth Military Medical University

摘　　要：目的：观察Ｊａｎｕｓｉｎ不同片段作为细胞外基质时对脊髓培养神经细胞形态的影响．方法：胚胎第１４日～１５日龄小鼠脊髓神经细胞接种在铺有原核表达的不同Ｊａｎｕｓｉｎ片段的培养孔内，在无血清培养液中培养２６ｈ，经ＡＢＣ法染色，图像处理仪摄取图像后，用体视学方法测量ＮＳＥ阳性细胞数及突起长度，计算单个细胞的突起长度并做统计学方差分析．结果：在无血清培养条件下，细胞呈不同形态：一种胞体较大、折光性差，突起具有复杂的分支，数量较多；一种胞体小、折光性强，多为单极或双极，突起分支少，数量较少；还有一种胞体扁平，自胞体向四周有较均匀的放射状突起，胞质内有２个～４个圆而亮的小泡状的结构，数量最少．结论：Ｊａｎｕｓｉｎ分子上的不同片段作为细胞外基质时，对脊髓培养神经细胞的突起生长影响不同，ＦＮ１－２组平均突起长度最短，明显抑制突起生长。Aim: To study the effects of different fragments of Janusin on morphological changes of neurons in vitro . Methods: The cell suspension were prepared from E14 15 mouse embryo spinal cord by mechanical dissection and digestion with enzyme. The cells were added to the culture dish absorbed different Janusin fragments expressed by E.coli . The cells were cultured in serum free medium for 26 h. The cells were then fixed and stained by ABC immunocytochemical method for NSE. The number of the cells and the length of the neurites were measured by stereological method using an image analysis. The result was analyzed statistically. Results: The cells grew well in the serum free medium for 26 h. Three kinds of cells could be identified: bright cells with single or two neurites; dark cells with well branched neurites; flat cells with 2￣4 round vesicles in the cell body. The cell number and the length of neurites could be influenced by different fragments of Janusin. It was found that FN1 2 inhibited neurite growth. Conclusion: Different motifs of Janusin used as substrate have different effects on cultured neural cells, adhesion or antiadhension, promotion or inhibition of the growth of neurites. They exert their influence through receptors on the cell membrane. This study may provide some clues to search for the different receptors which may play essential roles in neuronal development and plasticity.

关 键 词：Janusin 脊髓 细胞培养 体视学 神经细胞形态 

分 类 号：R338[医药卫生—人体生理学]