高铁酸钾对几种常见鱼类病原菌的杀菌效果测定  

THE GERMICIDAL EFFICACY OF POTASSIUM FERRATE(K_2FeO_4) AGAINST SEVERAL FISH-PATHOGENIC BACTERIA

作　　者：刘乾甫[1,2] 汪建国[1] 李明[3] 凌飞[1,2] 龚小宁[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]中国科学院研究生院,北京100049 [3]武汉工业学院饲料科学系,武汉430023

出　　处：《水生生物学报》2009年第5期818-825,共8页Acta Hydrobiologica Sinica

基　　金：国家支撑计划项目(2007BAD37B03);湖北省科技攻关计划(2007AA203A01);973计划项目(2009CB118700)资助

摘　　要：试验测定了高铁酸钾(K2FeO4)溶液对8种常见鱼类病原菌的MIC(最小抑菌浓度,minimal inhibitory concen-tration,MIC)和MBC(最低杀菌浓度,minimal bactericidal concentration,MBC),并研究了不同浓度的K2FeO4溶液对几种病原菌的杀灭效果。结果显示,K2FeO4对温和气单胞菌(Aeromonas sobria)、河弧菌(Vibrio flurialis)、弧菌I组淡水亚组弧菌(Vibriogroup I freshwater subgroup)的抑制效果良好;当各试验菌液浓度约为105cfu/mL时,施以K2FeO4溶液作用1h后,对以上两种弧菌亦表现出最优的杀灭效果;基于以上试验结果,选取这两种最为敏感的病原菌测定其长势随消毒剂浓度和时间的变化情况。研究对于使用K2FeO4浸泡消毒鱼体或全池泼洒消毒池塘时具有指导意义,并为使用K2FeO4防治鱼类弧菌病研究工作的展开打下基础。It has been well documented that potassium ferrate is a strong and environmental friendly oxidant. Under the acidic conditions, the redox potential of ferrate （ Ⅳ ） ions is higher than that of molecular ozone. The product of the ferrate （Ⅳ） oxidation reactions is considered to be the ferric hydroxide, which is a useful coagulant and can enhance the water remediation performance. Additionally, others disinfectant chemicals including chlorine and ozone, which are powerful and successful to combat the harmful micro-organism-causing problems, but such disinfectants have been confirmed to be of health concern. So, as a healthy potential alternative, potassium ferrate has been widely used in the fields of drinking water and wastewater treatmenti; however, there is no available report of its application to aquaculture. The purpose of this study is to investigate whether potassium ferrate can be used as an aquacultural disinfectant. Prior to this study, the concentration of potassium ferratc was determined by speetrophotometry. The equation of concentrations of Fe^2＋ against OD values was obtained： OD = 0. 005 ＋ 12241. 071 CFe^2＋ （ r = 0. 9985 ） , and then through transformation, the purity of potassium ferrate was 29.75%. In this study, eight kinds of fish-pathogenic bacteria were selected（Aeromonas sobria, Yersinia ruckeri, Aeromonas hydrophila, Vibrio flurialis, Aeromonas punctata subsp. Punctata, Pseudomonas fluorescent, Vibrio group I freshwater subgroup, Aeromonas punctata f. intedtinalis） , the MIC （ minimal inhibitory concentration, MIC ） and MBC （ minimal bactericidal concentration, MBC） of potassium ferrate against them were obtained, and the effect of potassium ferrate solution in different concentrations to sterilize these test germs were mensurated. The results showed that potassium ferrate had a better effect on inhibiting Aeromonas sobria, Vibrio flurialis, and Vibrio group I freshwater subgroup, and their MIC values were 1.2 mg/L. At the same time, the MBC values of the

关 键 词：高铁酸钾 (鱼类)病原菌 MIC MBC 杀菌效果 

分 类 号：S942[农业科学—水产养殖]