副猪嗜血杆菌和猪链球菌双重PCR方法的建立与应用  

Establishment and application of dulex PCR assay for identification of Haemophilus parasuis and Streptococcus suis

作　　者：贾爱卿[1] 李春玲[1] 王贵平[1] 杨冬霞[1] 何丽丽[1] 王金生[1] 

机构地区：[1]广东省农业科学院兽医研究所广东省兽医公共卫生实验室,广东广州510640

出　　处：《中国兽医学报》2009年第9期1155-1157,共3页Chinese Journal of Veterinary Science

基　　金：广东省农业攻关资助项目(2007A020300005-11);2005年关键领域重点突破招标资助项目(2005A10905006)

摘　　要：针对副猪嗜血杆菌和猪链球菌16S rRNA序列各设计1对特异性引物,分别能扩增出822 bp和294 bp的DNA片段,并据此建立了快速准确鉴别副猪嗜血杆菌和猪链球菌的双重PCR方法,临床试验证明该方法具有很好的特异性、敏感性,能对临床病料进行鉴别诊断。对161份临床样本检测结果显示,副猪嗜血杆菌的检出率为23.75%,猪链球菌的检出率为43.48%,二者混合感染率为9.94%。Haemophilus parasuis and Streptococcus suis were important pathogenes of swine both causing meningitis,arthritis,endocarditis,and it was difficult to distinguish them from the clinical symptom. In this study,the sequence of Haemophilus parasuis and Streptococcus suis 168 rRNA gene were amplified by polymerase chain reaction （PCR） ,and 822 bp and 294 bp specific DNA fragment could be amplified. Dulex PCR with the specific primer for differentiating Haemophilus purasuis and Streptococcus suis in pigs was established,and it was conformed to be specificity and sensitivity by clinical application. This method was applied in 161 diseased pig herds Haemophilus parasu is was 23.75% positive,and Streptococcus suis was 43. 48% positive.

关 键 词：猪链球菌 副猪嗜血杆菌 PCR 

分 类 号：S852.61[农业科学—基础兽医学] S855.99[农业科学—兽医学]