替米考星对内毒素炎症小鼠存活率和细胞因子的影响  

Effects of tilmicosin on cytokine response and survival rate of inflammatory mice by LPS

作　　者：何凤艳[1,2] 邓旭明[2] 刘明春[1] 宋宇[2] 张雪梅[3] 李乾学[2] 安娜[2] 黎宏宇[2] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]延边大学农学院,吉林延边133400

出　　处：《中国兽医学报》2009年第9期1186-1188,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30270999)

摘　　要：替米考星按20 mg/kg剂量经灌胃给药后,观察其对内毒素(LPS)炎症小鼠的保护率,并通过不同时间段眼球采血,分离血清,应用试剂盒用酶联免疫吸附分析(Enzyme-linked i mmunosorbent assy,ELISA)法测定小鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)、白细胞介素-6(IL-6)、白细胞介素-1(IL-1β)的水平。结果表明,替米考星明显提高了内毒素炎症小鼠的存活率,死亡率由100%降低为50%(P<0.05)。LPS(20 mg/kg)能提高血清中TNF-α、IL-10、IL-6和IL-1β的水平,替米考星能够显著抑制TNF-α、IL-6和IL-1β的释放,而明显提高IL-10的水平。The inflammation mice model was established by intraperitoneal injection of LPS（20 mg/kg）,and administered the tilmicosin （20 mg/kg） by gavage. The results showed the tilmieosin remarkably improved the survival rate of mice challenged with LPS. Compared with LPS group at 24 hour,the mortality of timilcosin group obviously dropped from 100% to 50% （P〈0.05）. There was a distinct decrease in serou TNF -α,IL-10,IL -6 and IL-1β contents and increase in serou IL- 10 levels in tilmicosin group compared with LPS group.

关 键 词：替米考星 内毒素 炎症小鼠 细胞因子 

分 类 号：S859.7[农业科学—临床兽医学]