钙信号与MDCK细胞活力的关系  

Relationship between calcium signals and viability of MDCK cells in vitro

作　　者：田勇[1] 乔键[1] 赵立红[1] 胡云峰[1] 金继昌[1] 栾智华[1] 王婷[1] 

机构地区：[1]中国农业大学动物医学院,北京100094

出　　处：《中国兽医学报》2009年第9期1189-1192,1196,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571398;30371063;30070567)

摘　　要：为探讨钙信号与犬肾上皮细胞(MDCK)活力之间的关系,采用MTT比色法观察了特异性钙调素拮抗剂-三氟拉嗪,胞内Ca2+螯合剂-BAPTA/AM以及Ca2+载体-A23187对MDCK细胞活力的影响。结果表明,12.5,25μmol/L三氟拉嗪和15μmol/L的BAPTA/AM作用72 h后分别使MDCK细胞活力降至对照组的51.4%,25.1%和34%,三氟拉嗪与BAPTA/AM对MDCK细胞活力的抑制作用随药物浓度增加和作用时间延长而增强;5μmol/L的A23187对MDCK细胞具有一定程度的刺激效应,但这种刺激效应随时间的延长而减弱;15μmol/L的BAPTA/AM可增强三氟拉嗪对MDCK细胞活力的抑制作用。说明Ca2+-钙调素系统在MDCK细胞生长和增殖过程中发挥重要作用。The objective of this study was to explore the relationship between calcium signals and viability of Mardin-Darby canine kidney （MDCK） cells in vitro. MTT colorimetric assay were used to detect the effects of specific calmodulin antagonists, trifluoperazine （TFP）, as well as Ca^2＋ chelators, BAPTA/AM and calcium ionophore A23187 on the viability of MDCK cells in vitro. The results showed that 12.5 μmol/L TFP,25 μmol/L TFP and 15 /μmol/L BAPTA/AM obviously decreased the viability of MDCK cells to 51.4% ,25.1% and 34% of the control after 72 hours, respectively. TFP and BAPTA/AM inhibited the viability of MDCK cells in a dose-and or time-dependent manner, the higher concentration and the longer time, the more significant the inhibition effects. A23187 （5 μmol/ L） stimulated the cell viability in a certain extent,but the stimulatory effect was decreased with prolonged time. The presence of 15 μmol/L BAPTA/AM obviously enhanced the inhibition of TFP on the viability of the MDCK cells. The experimental findings presented here suggest that Ca^2＋ CaM system may be engaged in in the development and proliferation of MDCK cells to modulate the cell viability and behavior.

关 键 词：钙调素拮抗剂 MDCK 三氟拉嗪 A23187 BAPTA/AM 

分 类 号：S852.2[农业科学—基础兽医学]