成年鸡原代肝细胞的原位分离和长期培养  

In situ isolation and primary long-term culture of adult chicken hepatocytes

作　　者：刘开永[1,2] 黄显会[1] 高海[1,3] 贺利民[1] 曾振灵[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]河南科技大学食品与生物工程学院,河南洛阳471003 [3]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国兽医学报》2009年第9期1193-1196,共4页Chinese Journal of Veterinary Science

基　　金：广东省自然科学基金资助项目(04105984)

摘　　要：为探讨成年鸡原代肝细胞的分离条件和长期培养过程中的形态学特征,本试验采用改进胶原酶二步原位灌流法分离高活性鸡原代肝细胞,并优化培养液,得到肝细胞长期培养的初步条件。结果显示,分离得到的活性肝细胞达94%,培养3 d细胞活性最强;贴壁培养时可见到肝细胞较为明显的分化过程。To investigate the sepraration method and morphological changes of long-term culture on primary adult chicken hepatocytes, the chicken primary hepatocytes were isolated by two-step in situ eollagenase perfusion method,the culture condition was optimized,and the primary compouds of culture solutions were tried. The results showed the viability freshly isolated chicken hepatocytes reached 94 %. Activity of the hepatocyte was highest at the third day. The morphology were effectively maintained in the culture medium. The research provided in vitro chicken hepatotcytes experimental platform for the animal husbandry and veterinary science.

关 键 词：成年鸡 原代肝细胞 二步灌流法 贴壁培养 

分 类 号：S852.167[农业科学—基础兽医学]