犬龈上牙结石的傅立叶红外光谱分析和扫描电镜观察  

SEM observation and FTIR analysis of canine dental salivary calculus

作　　者：孙卫东[1] 王金勇[2] 王小龙[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]河北工程学院动物科学系,河北邯郸057150

出　　处：《中国兽医学报》2009年第9期1222-1224,1228,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571366);江苏省高校"青蓝工程"优秀青年骨干教师资助项目(苏教2007-2)

摘　　要：应用傅立叶红外光谱分析、扫描电镜观察结合X-射线能谱分析方法对近年来从动物医院或养犬场收集到的63例患有犬牙结石自然病例的牙结石样品进行研究,观察了牙结石附着面和游离面的显微结构,并对其成分进行了分析。结果表明:(1)傅立叶变换红外光谱法可方便地鉴定出犬的牙结石的主要无机盐成分是碳酸羟基磷灰石。(2)扫描电镜结果显示,牙结石附着面存有微生物(细菌)先前占据留下的痕迹,其边缘有矿物沉积;牙结石游离断面的晶体成规则的片状或小圆柱状,牙结石游离面成不规则小团块状高度矿化区;由此推测犬牙结石的矿化启动区可能与微生物(细菌)有密切的联系。Sixty and three cases of canine dental calculus disease were obtained from some animal hospitals or dog farms in recent years. The composition of canine dental salivary calculus was analyzed by fourier transform infrared （FTIR） spectroscopy, X-ray energy dispersive spectrometer （EDS）,in the same time, the ultrastructural characteristics of the attached end and free end of the calculus was observed by scanning electron microscope（SEM）. The resuits indicated that： （1） The components of the calculus were satisfactorily identified by FTIR. （2） SEM observation showed holes on the attached end of the calculus,which may be attributed to spaces previously occupied by microorganism （bacteria）. Crystal showed regular lamination or leaf-like morphology in the free end and highly mineralized in the surface of the calculus. These predicted the process of dental salivary calculus biomineralization initiated phase may be tightly related to the debris of microorganism （bacteria）.

关 键 词：犬 龈上牙结石 生物矿化 傅立叶红外光谱 

分 类 号：S858.292[农业科学—临床兽医学]