肝再生过程中肾desmin和GFAP的表达以及MMPs的变化  

Changes of the Expression of Desmin and GFAP and the Activity of MMP-2 and MMP-9 in Kidney During Liver Regeneration

作　　者：张新胜[1] 程秋妍[1] 杜娟[2] 王雁北[2] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南师范大学体育学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2009年第5期112-115,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30270673);河南省高校骨干教师项目

摘　　要：采用免疫组织化学法和明胶酶谱电泳法研究大鼠肝部分切除(PH)后再生过程中肾结蛋白(desmin)和胶质纤维酸性蛋白(GFAP)的表达以及基质金属蛋白酶-2(MMP-2),-9(MMP-9)的活性变化.免疫组织化学结果显示,肝再生过程中肾小球系膜细胞、足细胞和间质细胞表达desmin和GFAP,而且二者的表达在PH后均经历了先减少后恢复的过程.明胶酶谱电泳结果显示,在对照组,检测到1条92 kD(pro MMP-9,MMP-9酶原形式)蛋白酶带,具有强的活性.在PH后8 d到14 d检测到了92 kD,86 kD(MMP-9),72 kD(pro MMP-2,MMP-2酶原形式)和66 kD(MMP-2)4条蛋白酶带.从第10 d到14 d,pro MMP-9和MMP-9活性逐渐增强,pro MMP-2和MMP-2活性逐渐降低.In this study, immunohistochemistry method and sodium dodecyl sulphate gelatin polyacrylamide gel electrophoresis （SDS-G-PAGE） are used to detecte changes of the expression of desmin and glial fibrillary acidic protein（GFAP） and the activity of matrix metalloproteinase-2（MMP-2） and matrix metalloproteinase-9 （MMP-9） in kidney respectively during liver regeneration after partial hepatectomy（PH）. The results show that： （1）Desmin and GFAP positivity in kidney is found in mesangial cells, podoeytes and mesenchymal cell,and the expression of desmin and GFAP is graduallly decreased from 6 h to 8 d, and then graduallly increased during liver regeneration; （2） At control group, the intensive activity of proMMP-9 is detected . At PH groups, the activities of proMMP 2, MMP-2 and proMMP-9, MMP-9 in kidney are detected from 8 d to 14 d , and the activities of proMMP-2 and MMP-2 are graduallly decreased, and proMMP-9 and MMP-9 are graduallly increased in kidney from 10 d to 14 d during liver regeneration.

关 键 词：部分肝切除 结蛋白 胶质纤维酸性蛋白 基质金属蛋白酶 

分 类 号：Q55[生物学—生物化学]