铜绿假单胞菌随机扩增多态性DNA基因分型及耐药性分析  

Random amplified polymorphic DNA technique of Pseudomonas aeruginosa and analysis drug-resistance

作　　者：肖辉[1] 张睢扬[1] 叶熊 李峰生[1] 李雪丽[1] 孙建华[1] 江其生[1] 董书魁[1] 王嘉漫 

机构地区：[1]第二炮兵总医院呼吸科,北京100088 [2]上海南汇区中心医院呼吸科,201300

出　　处：《国际呼吸杂志》2009年第17期1029-1032,共4页International Journal of Respiration

摘　　要：目的了解铜绿假单胞菌（Pseudomonas aeruginosa，PA）的基因分型及克隆菌株对耐药率的影响。方法对69株PA进行随机扩增多态性DNA基因分型和纸片琼脂扩散法耐药检测，结合基因分型结果进行耐药性分析。结果69株PA基因分型36型，有47株菌是克隆菌株。药敏实验结果显示，基因分型后PA对哌拉西林、头孢吡肟、头孢他啶、亚胺培南、美罗培南、头孢哌酮／舒巴坦、阿米卡星、环丙沙星、多黏菌素B、氨曲南的耐药率分别为27．3％、36．4％、27．3％、59．1％、54．6％、45．4％、31．8％、31．8％、0．0％、40．9％。结论在检测耐药率时应结合基因分型排除克隆菌株的影响，为当地提供合理的抗生素敏感性指南。Objective To investigate the genotypes of Pseudomona aeruginosa and the influence of colonizing strains to drug resistance. Methods Using randomly amplified polymorphic DNA technique for gene classifying and disk diffusion test for drug-resistance test classifying. Then, 69 isolated strains were analyzed for drug-resistance linking genotypes. Results Results demonstrated that genotypes were distinguished 36 and colonizing strains were 47 among the 69 Pseudomonas aeruginosa strains. A relatively high resistant rates of Pseudomonas aeruginosa strains to common antibiotics were found： pipenacillin 27.3% ,cefepime 36.4% ,ceftazidime 27.3% ,imipenem 59.1% ,meropenem 54.6% ,sulbactam/cefoperazone 45.4% ,amikacin 31.8%, ciprofloxacin 31.8%, polymyxin B 0.0%, aztreonam 40.9%. Conclusions These data suggest that we should be exclude colonizing strains linking genotypes in detecting the rate of drug resistance for developing appropriate antimicrobial susceptibility guidelines in local region.

关 键 词：铜绿假单胞菌 随机扩增多态DNA技术 细菌抗药性 基因型 

分 类 号：R446.5[医药卫生—诊断学] R378.991[医药卫生—临床医学]