犬胰腺迷走神经切断对胰岛素分泌第一时相的影响  

The influence of pancreatic vagotomy on first-phase insulin secretion in beagle dog

作　　者：石立[1] 成金罗[1] 李德[1] 沈默宇[1] 杨萍[1] 

机构地区：[1]南京医科大学附属常州市第二人民医院内分泌科,常州市糖尿病研究所,江苏常州213003

出　　处：《南京医科大学学报（自然科学版）》2009年第9期1232-1236,1250,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：常州市科技计划资助项目(CS2006215)

摘　　要：目的:探讨迷走神经在胰岛素分泌第一时相调节中的作用。方法:健康毕格犬5只,麻醉后开腹切断迷走神经腹腔干的前干和后干,在手术前和手术后行静脉葡萄糖耐量试验和持续颈动脉葡萄糖注入试验,分别于葡萄糖输注前空腹和输注后1、2、4、6、10min各从静脉取血样检测血糖、胰岛素。结果:手术后静脉葡萄糖耐量试验引起的胰岛素分泌第一时相较手术前降低且有统计学意义(P<0.05),而血糖较手术前无明显变化。小剂量葡萄糖持续颈动脉注入未引起体循环血糖变化,但可引起胰岛素分泌增加,2～4min达高峰;手术后胰岛素分泌降低且有统计学意义(P<0.05)。结论:中枢神经系统可能参与胰岛素分泌第一时相的调节,其可能是通过迷走神经途径。Objective：To investigate the effect of vagus nerve on first-phase insulin secretion. Methods：Five healthy beagle dogs were anesthetized and cut off anterior vagal trunk and post vagal trunk of vagal celiac trunk. Intravenous glucose tolerance test and the continuous infusion of glucose via the carotid artery were performed in five beagle dogs prior to and after truncal vagotomy. Blood samples were collected from jugular vein of opposite side each time at the 0,1,2,4,6 and 10 min to measure plasma insulin and blood glucose levels. Results： During intravenous glucose tolerance test, first-phase insulin secretion obviously dropped after truncal vagotomy compared to prior （P 〈 0.05）. But blood glucose failed to show significant difference before and after truncal vagotomy. The continuous infusion of low dose glucose via the carotid artery failed to induce any change in vein glucose level but caused an increasing of insulin secretion. It reached the peak at 2--4 min. Insulin secretion obviously dropped after truncal vagotomy （P 〈 0.05）. Conclusion： The central nervous system may play a role in regulating first-phase insulin secretion,which is mediated by vagus.

关 键 词：胰岛素分泌第一时相 迷走神经 中枢神经系统 

分 类 号：R335[医药卫生—人体生理学]