AG490对肥胖模型小鼠脂代谢及相关基因表达的影响  

Effects of AG490 on lipid metabolism of the obese mouse and expression of related genes

作　　者：谢亮[1] 孙超[1] 彭永佳[1] 堵斌[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第9期34-38,44,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30871785);国家"十一五"支撑计划项目(2006BAD04A11);教育部新世纪优秀人才计划项目(NCET-06-0865)

摘　　要：【目的】研究AG490阻断肥胖模型小鼠JAK2/STAT3信号通路对脂代谢及相关基因表达的影响。【方法】以雄性昆明小鼠为供试动物,构建肥胖模型。将肥胖模型小鼠分为2组,处理组用JAK2特异性抑制剂AG490(1 mg/(kg.d))腹腔连续注射2周,对照组腹腔连续注射相同剂量的生理盐水。2周后处死小鼠,检测各组小鼠血清中总胆固醇(TC)、总甘油三酯(TG)、高密度脂蛋白固醇(HDL-C)的含量;取脂肪组织提取总RNA,RT-PCR方法检测JAK2/STAT3信号通路基因(JAK2、STAT3)及脂代谢关键基因(FAS、PPARγ、HSL)的表达量。【结果】与对照组相比,AG490可显著降低小鼠血清中的HDL-C水平;与对照组相比,处理组脂肪组织中JAK2 mRNA表达量差异不显著(P>0.05),STAT3 mRNA表达量显著降低(P<0.05),脂代谢相关基因FASmRNA表达量极显著降低(P<0.01),PPARγmRNA表达量显著降低(P<0.05),HSLmRNA表达量差异不显著(P>0.05)。【结论】JAK2/STAT3通路可能通过影响脂肪组织中生脂基因的表达而调节体脂沉积。[Objective] It aimed to study the effects of AG 490 on lipid deposition and expression of relevant genes of the obese mouse by JAK2/STAT3 signal transduction pathway occlusion. [Method] The obese model which was constructed by the male Kunming mouse were divided into 2 groups. The treatment group was treated with intraperitoneal injection by AG490 （1 mg/（kg ·d）） and the control group was treated by the same method with NS （1 mg/（kg · d）） for two weeks,then executed and the adipose was reserved. To estimate the content of TC,TG and HCL-C in blood,Total RNA was extracted. RT-PCR was used to analyze the expression of JAK2/STAT3 signal transduction pathway and adipogenesis genes （FAS, PPARγ and HSL）. [Result] Compared with the control group, AG490 was able to cut down the level of HDL-C significantly in blood with the treated mice;Compared with the control group, the mRNA expression of JAK2 had not significant difference （P〉0.05） and the mRNA expression of STAT3 was significantly lowered （P〈0.05） in the treatment group. The mRNA expression of FAS was significantly lower （P〈0.01）,the mRNA expression of PPARγ had a significant reduction （P〈0.05） and the mRNA expression of HSL had no significant difference （P〉0.05）. [Conclusion] We conjectured that JAK2/STAT3 signal transduction pathway regulated lipid deposition by affecting the expression of adipogenesis genes in adipose tissue.

关 键 词：JAK2 STAT3 AG490 肥胖模型 脂代谢 

分 类 号：Q493.5[生物学—生理学]