日粮精粗比对荷斯坦公犊瘤胃组织结构的影响  

Effect of different dietary concentrate to forage ratio on rumen morphological structure of Chinese Hostein bull

作　　者：张双奇[1] 昝林森[1] 梁大勇[1] 桂林生[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第9期59-64,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十一五"科技支撑计划项目(2006BAD04A11);陕西省重大科技专项(2006KZ07-G1)

摘　　要：【目的】探讨不同日粮精粗比对荷斯坦公犊瘤胃组织结构的影响。【方法】选择9头6月龄体质量相近的荷斯坦公犊,随机分为Ⅰ、Ⅱ、Ⅲ组,分别饲喂m(精料)∶m(粗料)为4∶6,5∶5和6∶4的日粮,粗料为苜蓿干草和玉米青贮料,至9月龄屠宰,采集瘤胃组织块,制备石蜡切片,对瘤胃4个不同部位(前背、后背、前腹、后腹)进行组织形态学观察。【结果】瘤胃黏膜上皮角化层、颗粒层和基底层的厚度随着精料比例的增加而增大,其中Ⅰ组和Ⅲ组之间差异显著(P<0.05);瘤胃固有膜厚度在3组犊牛之间差异不显著(P>0.05),瘤胃乳头长度和密度随精料比例的增加而增大,Ⅰ组和Ⅲ组之间差异不显著(P>0.05);乳头宽度则随精料比例的增加而减小,Ⅰ组和Ⅲ组之间差异不显著(P>0.05)。【结论】日粮精粗比对中国荷斯坦公犊瘤胃黏膜上皮角化层、颗粒层和基底层的厚度均有显著影响(P<0.05),而对固有膜厚度、瘤胃乳头长度、宽度和密度均无显著影响(P>0.05)。[Objective] The research was conducted to study the effect of different levels of forage to concentrate ratio on rumen morphological structure of Chinese Hostein bull. [Method] Nine 6-month-old Chinese Hostein bulls were chosen at similar birth weight to divide into three groups （Dietary forage to concentrate ratio were 4 ： 6,5 ： 5,6 ： 4 respectively）, the crude fodder contained alfalfa hay and corn silage, and were slaughtared three months later. Histomorphology of four different parts of rumen （including saccus cranialis, saccus cecus caudodorsalis, recessus ruminis, saccus cecus caudoventralis） were observed by means of paraffin method. [Result] The results indicated that the epithelium horny, stratum granulosum and stratum basale were increased with the increase of forage to concentrate ratio, and the differences among group Ⅰ and group Ⅲ were significant （P〈0.05）. There were no significant differences among the three groups of membrana propria（P〉0.05）. The length and the density of rumen papillae were increased with the increase of forage to concentrate ratio,and the differences among group Ⅰ and group Ⅲ were not significant（P〉0.05）. The width of tureen papillae decneased with the increase of forage to concentrate ratio,and the differences among group Ⅰ and group Ⅲ were not significant（P〉0.05）. [Conclusion] Different levels of forage to concentrate ratio had significant effects （P〈0. 05） on epithelium horny, stratum granulosum and stratum basale,while there were no significant effects （P〉0.05） on membrana propria,as well as the length,the width and the density of rumen papillae.

关 键 词：日粮精粗比 中国荷斯坦公犊 瘤胃 组织形态 

分 类 号：S826.91[农业科学—畜牧学]