睾酮与雄激素受体在中国林蛙精巢中的免疫细胞化学定位  

Immunocytochemical localization of testosterone and androgen receptor in testis of Rana chensinensis

作　　者：杨纯[1,2] 张育辉[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]山西师范大学生命科学学院,山西临汾041004

出　　处：《陕西师范大学学报（自然科学版）》2009年第4期67-71,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基础研究计划项目(2001SM26);山西师范大学自然科学基金项目(YZ06016)

摘　　要：用免疫细胞化学方法检测了睾酮(T)和雄激素受体(AR)在中国林蛙生精周期中不同时期精巢内的表达定位.结果显示:在中国林蛙生精周期的Ⅰ～Ⅴ期,T和AR在精原细胞、精母细胞、精子细胞、精子、支持细胞和间质细胞内均有分布.在不同时期的精巢中,T和AR在生精细胞的定位具有一致性.在Ⅱ期,精子细胞内T阳性反应和AR阳性表达最强.在生精周期的Ⅰ～Ⅴ期,支持细胞内T的阳性反应强度无明显变化.在Ⅴ期,支持细胞内AR的阳性表达强度显著高于其他各期,而间质细胞内AR的阳性表达最弱.结果表明,T在中国林蛙精巢内细胞的分化和发育过程中的作用具有高度可变性,T调控中国林蛙精巢的功能和发育.Localisation of testosterone（T）and androgen receptors（AR）was investigated in different stages of the spermatogenetic cycle of Rana chensinensis,using immunocytochemistry.The results showed that both T and AR are distributed in spermatogonia,spermatocytes,spermatids,spermatozoa,Sertoli and Leydig cells from stage Ⅰ to stage Ⅴ of spermatogenesis.A strong positive distribution of T and AR was observed in spermatids in stage Ⅱ.Expression of T in Sertoli cells no significantly changes in intensity from stage Ⅰ to stage Ⅴ of spermatogenesis.In stage Ⅴ,expression of AR in Sertoli cells was higher than that in other stages.Nevertheless,expression of AR in Leydig cells was lower in stage Ⅲ than that in other stages.The results suggested that the roles of T in the differentiation and development of the spermatic cells were highly diverse.There is a T regulation of the function and development of the frog testis.

关 键 词：中国林蛙 精巢 睾酮 雄激素受体 免疫细胞化学 

分 类 号：Q959.53[生物学—动物学] Q492