慢性束缚应激性抑郁与雌性小鼠雌激素和海马雌激素受体的关系  

Relationship of depression-like behavior induced by chronic restraint stress estrogen and hippocampal estrogen receptor of female mice

作　　者：李庆娇[1] 厉蔚[1] 安书成[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2009年第4期82-87,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基金资助项目(2006C240)

摘　　要：采用慢性束缚应激抑郁模型,通过强迫游泳测试(Forced swimming test,FST)和免疫组织化学方法,探讨了慢性束缚应激对雌性小鼠海马雌激素受体(Estrogen receptor,ER)和行为表现的影响.结果显示:与对照组相比,慢性束缚应激小鼠的体重降低或增长速度明显减慢,FST不动时间增长(P<0.01),ER表达下降(P<0.01);卵巢切除后的小鼠在行为表现和ER表达与慢性束缚应激组的结果相似;与应激组相比,应激同时周期性外源补充雌激素,小鼠体重增长速度加快,FST不动时间明显减少(P<0.01),而ER表达明显下降(P<0.01).结果表明,慢性束缚应激能诱发抑郁,慢性束缚应激性抑郁发生与雌激素水平及雌激素受体下降有关.To observe the effects of chronic restraint stress（CRS）on the expression of estrogen receptor（ER）in hippocampus of female and behavioral changes,female mice were selected,the animal depression model related chronic restraint stress was used,and forced swimming test（FST）was carried out to observe their behaviors.Immunohistochemistry was used to observe the expression of ERα and ERβ in hippocampus.Chronic restraint stress could notably restrain the augment of animal weights and increase their immobility time in FST.Compared with control group,there were significant reduction in the number of ERα-IR cells,ERβ-IR cells in hippocampus in CRS group.OVX could also notably restrain the augment of animals weight and increase their immobility time in FST.Compared with control group,there were significant increase in the number of ERα-IR cells in the DG,and significant reduction in that of ERβ-IR cells in the CA1,CA2,CA3 in OVX group.During the course of chronic restraint stress,female mouse were injected with E periodically,and we found that animal weights were notably increased and their immobility time was reduced remarkably compared with Stress group.Additionally,there were significant reductions in the number of ERα-IR cells and ERβ-IR cells in the hippocampus.Chronic restraint stress can lead to depression-like behavior,and there is a close relationship between stress-induced depression and estrogen.

关 键 词：雌激素 慢性束缚应激 抑郁 海马 

分 类 号：Q189[生物学—神经生物学] R749.4[生物学—普通生物学]