核磁共振碳谱对昆布多糖硫酸酯的结构分析  

Structural Analysis on Preparation of LAMS from Laminarin by ^(13) C NMR

作　　者：张爱凤[1] 邹明畅[2] 崔飞伦[2] 倪鸿昌[2] 

机构地区：[1]镇江市第四人民医院,江苏镇江212002 [2]江苏大学附属人民医院,江苏镇江212002

出　　处：《现代中药研究与实践》2009年第4期35-36,共2页Research and Practice on Chinese Medicines

基　　金：镇江市科学技术局资助项目(项目编号:SH2007027)

摘　　要：目的昆布多糖进行硫酸酯化后,对产物结构进行分析,为研究其生物活性奠定物质基础。方法利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化,利用核磁共振碳谱研究其结构。结果13C-NMR谱显示多糖的2位羟基为SO42-的主要取代位置。结论利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化,昆布多糖的2位羟基为SO42-的主要取代位置。Objectives To sulfate the laminarin, then analyze the structure of polysaccharide sulfate, so as to make a foundation for the further research about its biological activity. Methods LAMS was prepared by chlorsulfonic acid - pyridine method ,and the structure of was analyzed by ^13 C NMR. Results The most of the SO4^2- of LAMS were on 2-OH by ^13C -NMR. Conclusion LAMS was prepared from laminarin by chlorsulfonic acid - pyridine method. The most of the SO4^2- of LAMS were on 2-OH by ^13C -NMR.

关 键 词：昆布多糖 硫酸酯化 核磁共振碳谱 结构分析 

分 类 号：R284.1[医药卫生—中药学]