类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响  

Effects of Porcine Circovirus-like Agent P1 Infection on Counts and Proliferation Activity of T Lymphocyte in Peripheral Blood of Piglets

作　　者：温立斌[1] 何孔旺[1] 杨汉春[2] 郭容利[1] 钟书霖[1] 周俊明[1] 陈梦海[3] 

机构地区：[1]江苏省农业科学院兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏南京210014 [2]中国农业大学动物医学院,北京100094 [3]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《华北农学报》2009年第B08期1-4,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家“973”计划前期研究专项基金(2007CB116308);江苏省自然科学基金(BK2008351)

摘　　要：探讨类猪圆环病毒因子P1感染对猪细胞免疫功能的影响。将12头30日龄广西巴马小型猪随机分成试验组和对照组,每组6头。在猪感染P1后第3,8,17,24,31和40天,采用血常规和单色流式细胞术分析猪外周血T淋巴细胞的含量,通过WST法对其增殖活性进行检测。P1感染组的T淋巴细胞数量(绝对数和相对数)整体趋势低于对照组,而两组的T淋巴细胞增殖活性没有显著差别。P1感染对机体的细胞免疫功能有一定程度的影响。The research is to explore the effects of porcine circovirus-like agent PI infection on counts and prolifera- tion activity of T lymphocyte in peripheral blood of piglets. Twelve 30-day-old guangxi bama miniature pigs were randomly divided into two groups of six animals each. A complete haemogram and single-color flow cytometry was performed to de- termine the T lymphocyte of 3,8,17,24,31 and 40 days after inoculation, meanwhile, WST assay was to do the T lympho- cyte proliferation activity. There was no significant difference in T lymphocytes proliferation activity between the two groups of pigs（ P 〉 0.05）. On the other hand, in comparison to control pigs, the means of T lymphocyte percentages and absolute counts were overall lower in pigs infection with P1 .Altogether,the results would suggest that P1 infection has a certain degree of influence to mount an effective cell mediated immune response.

关 键 词：类猪圆环病毒因子 广西巴马小型猪 T淋巴细胞计数 T淋巴细胞增殖活性 

分 类 号：S852.65[农业科学—基础兽医学]