抗伪狂犬病毒闽A株单克隆抗体的制备及鉴定  

Preparation and Characterization of Monoclonal Antibody to PRV-FA Strain

作　　者：吕蔚[1] 李潭清[1] 韩新影[1] 康亚男[1] 杨润德[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000

出　　处：《华北农学报》2009年第B08期27-29,共3页Acta Agriculturae Boreali-Sinica

摘　　要：用纯化的猪伪狂犬病病毒闽A株(PRV-FA)免疫Balb/c鼠,取脾细胞和骨髓瘤细胞进行细胞融合,经间接ELISA筛选,获得2株能稳定分泌伪狂犬病毒单克隆抗体的杂交瘤细胞株,分别命名为4G9E9和4H9C9。这2株杂交瘤细胞株细胞培养上清和小鼠腹水效价(ELISA)分别为1∶6400、1∶12800及1∶12800、1∶25600。特异性鉴定结果表明,各株单抗均不与猪瘟病毒、乙脑病毒、猪呼吸繁殖障碍综合症病毒、猪细小病毒等发生交叉反应,这2株抗PRV杂交瘤细胞株的获得为进一步建立准确快速的抗原检测方法奠定了基础。The purified psudorabies virus strain FA（PRV-A）was inoculated to Balb/c mice. Two m-onoclonal hybri- dona cell named 4G9E9 and 4HgG9 lines which were able to stably excrete monoclonal antibodies against PRV were pro- duced by means of hybridoma technique and indirect ELISA detection. The indirect ELISA titer of culture supernatant and ascites were 1：6 400,1 ： 12 800 and 1 ： 12 800,1：25 600 .All these monoclonal antibodies didn＇t react with HCV,JEV, PRRSV and PPV. These results could be used to develop diagnostic methods for detection of PRV antigen as well as to in- vestigate protein function of PRV.

关 键 词：伪狂犬病病毒 单克隆抗体 制备 

分 类 号：Q785[生物学—分子生物学]