根癌农杆菌介导小麦成熟胚的遗传转化研究  

Studies on Wheat Genetic Transformation Mediated Via Agrobacterium-tumefaciens in Which the Mature Embryos to be Explants

作　　者：郭丽[1] 谷俊涛[2] 龙素霞[1] 郭程瑾[1] 肖凯[1] 

机构地区：[1]河北农业大学农学院,河北保定071001 [2]河北农业大学生命科学学院,河北保定071001

出　　处：《华北农学报》2009年第B08期54-57,共4页Acta Agriculturae Boreali-Sinica

基　　金：科技部"973"前期项目(2007CB116209)资助;河北省重点基础研究项目(08965525D)

摘　　要：以小麦成熟胚为转化受体,采用农杆菌介导的遗传转化技术,建立了小麦的转基因株系。结果表明,建立的小麦遗传转化和植株再生体系,在选择压下由侵染的小麦成熟胚中,获得的抗性愈伤组织频率为57.50%,愈伤组织分化频率为8.58%,植株分化比例为0.83%。对再生植株中报告基因Gus的PCR检测结果表明,供试3株小麦的PCR均为阳性。与未进行遗传转化的对照植株相比,再生植株的Gus活性明显提高。表明再生的供试植株均为转基因植株。因此,本研究建立的小麦成熟胚遗传转化和再生体系,为今后小麦的高频转基因系的获得提供了参考依据。In this study, the genetic transformation procedure of wheat mediated via Agrobacterium-tumefaciens in which the mature embryos to be the explants was established. Under selection pressure condition, the generation rate of re- sistant ealli to the transformed total mature embryos was 57.50% ,with a differentiation rate of resistant buds to the trans- formed total mature embryos being 8.58 %, and a plant generation rate of generated plants to the transformed total mature embryos being 0.83 %. In the tested three generated plants, PCR amplifications for the reporter gene C, us were all posi- tive. In the meantime, the Gus activities in the plants were all significantly higher than the control （non-transformed plants）. These results demonstrated that the tested plants generated from the transformed mature embryos were transgenie. Therefore, the genetic transformation approach established in this study would provide a powerful method for establishment of transgenie wheat lines in the future.

关 键 词：小麦(Triticum AESTIVUM L.) 成熟胚 遗传转化 分子鉴定 

分 类 号：S512.03[农业科学—作物学]