玉米弯孢菌叶斑病菌致病性分化的RAPD鉴定  

Study on RAPD for Physiological Differentiation of Curvularia lunata in Maize

作　　者：鄢洪海[1] 

机构地区：[1]青岛农业大学植物保护学院,山东青岛266109

出　　处：《华北农学报》2009年第B08期258-261,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家"九五"科技攻关项目(96005010303);青岛市自然科学基金项目(05-JC-55)

摘　　要：从60条随机引物中选择12对组合，对玉米弯孢叶斑病菌基因组DNA进行随机扩增，共扩增出128条遗传标记。根据这些标记使用Statistic统计软件对菌株之间相互关系进行聚类分析，结果表明：菌株间最高相似系数是为87％，最低为47％。22个供试菌株在连锁距离0．4范围内全部聚在一起，树状图上的菌株可分成5—6组。a组包括：98-36、98．25、98-17、98-07、98-01和98-286个菌株，是遗传基础最接近的一组。One hundred and twenty-eight piece of belt signs are multiplied through selecting 12 pairs of associations form 60 piece of random decoy and multiplying Curvularia lunata randomly. According to the signs, pathogens mutual re- lations are analyzed. The result is that the highest similarity degree of the pathogen is 87 % and the lowest is 47 %. Twen- ty-two experimental pathogen all gather together by all stems on 0.4 to the stem in the linked together. The pathogen forming tree shape can be divided into 5 or 6 groups. Group a consists of 6 pathogen,they are 98-36,98-25,98-17,98- 07,98-01 and 98-28,which is the most approximate one on heredity base.

关 键 词：玉米弯孢叶斑病菌 致病性分化 DNA 多态性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]