实验性大肠癌模型的建立及中药的干预作用  

Established colorectal carcinoma model in rats to investigate anti-colorectal carcinoma activities of Zuojin Pill(左金丸) and Fanzuojin Pill(反左金丸)

作　　者：文彬[1] 黄秋凌[1] 熊旻利[2] 戴伟怡 梁齐桁[2] 刘玲[2] 龚艳青[1] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405 [2]广州中医药大学第二临床医学院,广州510405

出　　处：《中药药理与临床》2009年第4期79-82,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(No:30400602;30672687)资助

摘　　要：目的:用两种不同方法建立大肠癌实验动物模型,在肿瘤发生发展过程中,研究左金丸与反左金丸这两种寒热相反的药物对肿瘤进程的干预作用,寻找研究中药防治大肠癌恰当的实验动物模型。方法:人工条件下培养人大肠癌HT29细胞,建立BALB/C裸鼠荷瘤大肠癌模型;用不同浓度的DMH(1,2-二甲基酰肼)诱导W istar大鼠大肠癌模型。在模型建立过程中,用左金丸和反左金丸进行干预,取瘤体组织进行组织病理学评价,观察中药对模型病程发展的干预作用。结果:用BALB/C裸鼠荷瘤大肠癌模型难以观察到肿瘤发生过程中药的干预作用;在高浓度的DMH(35mg/kg)诱导下也不能显著地观察到两对中药对癌症发生过程的干预作用;但在较低浓度下(25mg/kg),左金丸在肿瘤发生的早期具有良好的阻碍大肠恶变的作用,反左金丸的作用次之。结论:观察药物对肿瘤早期的干预作用,致癌剂的浓度不宜太大,DMH 25mg/kg是研究左金丸和反左金丸对早期大肠癌干预作用模型建立较适宜的致癌剂浓度。Objective： To establish colorectal carcinoma model in rats and investigate the intervention of Zuojin Pill and Fanzuojin Pill on the progressive colorectal carcinoma.Methods： The nude mice transplanted HT29 cells and the DMH induced rat colorectal carcinoma model were established.Nude mice and rats were randomly divided into Zuojin pill group,Fanzuojin pill group and control group.Nude mice were killed after 5 weeks treatment,tumor size was detected.Rats were randomly divided into two groups： A（DMH： 35mg/kg）and B （ DMH ： 25mg/kg）, A and B were randomly divided into Zuojin pill group, Fanzuojin pill group and control group respectively. A and B killed respectively after 11, 21,24, 34 weeks＇treatment. To observe the schedule of tumor development effected by Zuojin pill and Fanzuojin pill. Results： Zuojin pill and Fanzuojin pill can repress the invasion and development of the early rat carcinoma induced by DMH（25mg/kg） effectively, while in late cancer, this action is not obvious. In nude mice model and rat carcinoma induced by DMH（30mg/kg）, this action is not obvious. Conclusion： Zuojin pill and Fanzuojin pill can effectively repress the invasion and development of the early rat carcinoma induced by DMH（25mg/kg）.

关 键 词：大肠癌 裸鼠 1 2-二甲基酰肼 左金丸 反左金丸 

分 类 号：R285[医药卫生—中药学]