海水浸泡降低血管内皮细胞迁移能力并抑制血管生成  

Seawater immersion depresses the migration of vascular endothelial cell and inhibits angiogenesis

作　　者：熊鸣[1] 曲佳[1] 周兰萍[2] 张达矜[1] 乔媛媛[1] 胡明[1] 赵晓航[1,2] 

机构地区：[1]中国人民解放军海军总医院基础医学研究中心,北京市100048 [2]中国医学科学院北京协和医学院肿瘤研究所分子肿瘤学国家重点实验室,北京市100021

出　　处：《世界华人消化杂志》2009年第21期2152-2158,共7页World Chinese Journal of Digestology

基　　金：总后医药卫生科研计划基金资助项目;No.06MB022;海后司令部科研计划基金资助项目;No.07-3309;国家重点基础研究发展计划"973"基金资助项目;No.2004CB518707~~

摘　　要：目的:观察海水浸泡对血管内皮细胞迁移和血管生成的影响,为探讨海水致伤机制、制定科学救治原则提供实验依据.方法:建立ECV304细胞划痕创伤模型,分别给予生理盐水和人工海水浸泡处理,划痕创伤愈合修复分析观察细胞迁移能力;建立人食管鳞癌EC0156裸鼠皮下移植瘤模型,分别给予生理盐水和人工海水干预,监测肿瘤体积并绘制肿瘤生长曲线,观察肿瘤边缘血管的生成,免疫组织化学染色检测肿瘤组织内微血管密度.结果:海水浸泡后,ECV304细胞失去正常形态,并随浸泡时间的延长,呈明显"脱水"状态.在划痕后17h,对照组划痕已基本愈合,而实验组仍可见清晰划痕,且以实验4h组划痕尤其明显.与对照组相比,实验组细胞划痕愈合明显延迟,细胞迁移能力降低;与对照组相比实验组肿瘤边缘新生血管较少,前者肿瘤边缘新生血管较丰富,两者有显著性差异(微血管密度值:1.2±0.44vs3.2±0.83,P<0.05).实验组肿瘤生长缓慢,肿瘤体积较小(P<0.05).结论:海水浸泡能够降低血管内皮细胞迁移能力并抑制血管生成.AIM：To investigate the effects of seawater immersion on vascular endothelial cell migration and angiogenesis.METHODS：A scratch wound model was established using ECV304 cells.Cells were divided into control group（immersed in normal saline）and experimental group（immersed in seawater）.Cell migration was examined using the scratch wound healing recovery assay.A nude mouse xenograft model was developed by subcutaneous inoculation of esophageal squamous cells（ECO156）in nude mice.Tumors were also divided into conttrol group （immersed in normal saline） and experimental group （immersed in seawater）. Tumor volume and weight were measured, and tumor growth curves were plotted. Neovascular vessels surrounding the tumor were observed, and microvessel density was detected by immunohistochemical staining. RESULTS： After seawater immersion, ECV304 cells lost their normal morphology, and showed obvious dehydration with the prolongation of immersion duration. Seventeen hours after wounding, the scratch wound in the control group had almost completely healed, while that in the experiment group was still obvious, suggesting that the healing of scratch wound in the experiment group was delayed significantly and the migration of cells was depressed. The microvessel density in the experiment group was significantly lower than that in the control group （1.20 ± 0.44 vs 3.20 ± 0.83, P 〈 0.05）. Compared with the control group, tumors in the experiment group grew slowly and had significantly smaller size （P 〈 0.05）. CONCLUSION： Seawater immersion may depress the migration of vascular endothelial cells and inhibit angiogenesis.

关 键 词：海水浸泡 血管内皮细胞 血管生成 细胞迁移 

分 类 号：R543[医药卫生—心血管疾病]