针刺对便秘模型大鼠PGP9.5表达的影响  

Effect of acupuncture on PGP 9.5 expression in rats with experimental constipation

作　　者：丁曙晴[1,2] 丁义江[2] 季新涛[1] 韩惦梅[3] 孙明明[1] 霍维宇[1] 黄书亮[3] 耿建祥[3] 

机构地区：[1]南京中医药大学研究生部,江苏省南京市210046 [2]南京中医药大学第三附属医院全国中医肛肠医疗中心,江苏省南京市210001 [3]南京中医药大学第三附属医院病理科,江苏省南京市210001

出　　处：《世界华人消化杂志》2009年第21期2184-2187,共4页World Chinese Journal of Digestology

基　　金：江苏省中医药局基金资助项目;No.HL07061~~

摘　　要：目的:观察针刺对便秘模型大鼠肠神经系统神经元标志物PGP9.5表达的影响.方法:将60只大鼠按照体质量及随机分组.正常对照组15只,45只大鼠予生大黄水煎液灌胃,开始用量为100g/(kg?d),最后为1280g/(kg?d),45d后造模36只,治疗前处死12只作为造模末治疗前组对照,剩余随机分为针刺组和空白治疗组,各12只.针刺组大鼠针刺天枢和足三里,天枢加电针,疏密波刺激10min,每日1次,共计14d;空白治疗组不给予治疗.2组14d后处死,取距离肛门5cm肠管,用HE染色和PGP9.5免疫组织化学染色,采用Image-Pro Plus 5.0计算各组PGP9.5的综合吸光度值(IA值)分析肠神经节细胞的状况.结果:正常组PGP9.5表达的IA值(×104)为47.38±9.04,治疗前组为20.36±9.12,2组比较有显著差异(P<0.000);空白组为28.51±9.43,与治疗前组比较无统计学差异(P>0.05);针刺组为41.39±19.56,与治疗前组和空白组比较均有统计学差异(均P<0.05),与正常组比较无统计学差异(P>0.05).结论:针刺有助于改善大黄水煎液灌胃泻剂便秘模型大鼠神经节细胞功能.AIM：To investigate the effect of acupuncture on the expression of PGP 9.5（a neuronal marker in the enteric nervous system）in rats with experimental constipation. METHODS：A total of sixty rats were used in the study,of which 15 rats were included in normal control group and the remaining 45 rats were in-tragastrically administered with Rhubarb Decoction to induce experimental constipation.The resulting 36 model rats were equally divided into model control group,sham treatment group and acupuncture treatment group.Rats in the model control group （n = 12） were killed before treatment, while those in the acupuncture treatment group and sham treatment group were subjected to electroacupuncture or sham treatment for 14 days and killed. The intestinal canal about 5 cm away from the anus was taken. HE staining and immunohistochemicaI staining were then performed to determine the integrated absorbance （IA） of PGP9.5 staining.RESULTS： The IA value was significantly higher in the normal group than the model control group （47.38 ± 9.04 vs 20.36±9.12, P 〈 0.000）. No significant difference was noted in the IA value between the sham treatment group and the model control group （28.51 ± 9.43 vs 20.36 ±9.12, P = 0.200）. The IA value was significantly higher in the acupuncture treatment group than the model control group and sham treatment group （41.39± 19.56 vs 28.51 ±9.43 and 20.36 ±9.12, P = 0.021 and 0.002, respectively） though no significant difference was observed between the normal control group and the acupuncture treatment group （P = 0.297）. CONCLUSION： Acupuncture can help improve the function of intestinal ganglion cells in rats with Rhubarb Decoction-induced slow-transit constipation.

关 键 词：结肠慢传输性便秘 模型 肠神经系统 肠神经元 PGP9.5表达 

分 类 号：R245[医药卫生—针灸推拿学]