表面氧化镍钛形状记忆合金的细胞毒性  

Cytotoxity of nickel-titanium shape memory alloys following surface oxidation

作　　者：张雪[1] 张扬[1] 冯翠娟[1] 王强[2] 

机构地区：[1]中国医科大学口腔医学院正畸科,辽宁省沈阳市110002 [2]中国医科大学口腔医学院口腔材料教研室,辽宁省沈阳市110002

出　　处：《中国组织工程研究与临床康复》2009年第34期6645-6649,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：辽宁省自然科学基金资助项目(20082088)~~

摘　　要：背景:对镍钛形状记忆合金进行表面改性可以减少镍离子的析出,达到提高生物相容性的目的。目的:在镍钛形状记忆合金表面制备氧化涂层,评价表面氧化镍钛形状记忆合金对细胞毒性的影响。设计、时间及地点:材料-细胞学体外实验,于2008-11/12在中国医科大学口腔医学院中心实验室完成。材料:L-929细胞由中国医科大学中心实验室提供。镍钛形状记忆合金板材为北京有研亿金新材料股份有限公司产品。方法:将镍钛形状记忆合金板材制备成半径10mm、厚度3mm的圆形合金试件,分为2组:氧化组合金表面进行氧化处理,即酸洗,丙酮中超声波清洗,空气干燥,在沸腾的H2O2溶液中氧化2h,取出后去离子水超声波清洗;非氧化组合金表面不进行氧化处理。合金浸提液的制备按2组合金试件表面积与培养液之比为3cm2/mL加入RPMI-1640培养液,置于37℃培养箱内96h,微孔滤膜除菌后备用。并设立阳性对照组,加入6.4%苯酚培养液;空白对照组,加入1640培养液。主要观察指标:倒置相差显微镜观察L-929细胞在合金浸提液中的生长情况,通过MTT试验得出细胞在培养第24,48h的吸光度值,对表面氧化镍钛形状记忆合金做出细胞毒性评价。结果:①培养24h,阳性对照组细胞形态异常;氧化组、非氧化组、空白对照组细胞贴壁生长状态良好。培养48h,阳性对照组许多细胞呈中毒形态;氧化组、空白对照组细胞形态正常,生长旺盛;非氧化组少量细胞核固缩。②与空白对照组比较,随培养时间延长氧化组、非氧化组吸光度值均相应增加(P<0.05),且氧化组增加幅度高于非氧化组(P<0.05)。氧化组合金在培养24,48h时细胞毒性均为0级,表现出轻微的细胞毒性;非氧化组合金培养24h时细胞毒性为0级,48h时细胞毒性为1级,表现出较明显的细胞毒性;空白对照组无细胞毒性。结论:镍钛形状记忆合金经过表面氧化处理后细胞毒�BACKGROUND： Surface modification on nickel-titanium shape memory alloy （NiTi-SMA） may reduce separation of nickel ions so as to enhance the biocompatibility. OBJECTIVE： To evaluate the cytotoxicity of NiTi-SMA with surface oxidation. DESIGN, TIME AND SETTING： An in vitro study based on materials and cytology level was performed at Central Laboratory, School of Stomatology, China Medical University from November to December 2008. MATERIALS： L-929 cells were provided by Central Laboratory of China Medical University, and NiTi-SMA was provided by Grikin Advanced Materials Co., Ltd., Beijing. METHODS： NiTi-SMA was made into round samples with radius 10 mm and thickness 3 mm which were divided into oxidation group and non-oxidation group. Samples in the oxidation group were ultrasonically rinsed with acetone, dried in the air, oxidated in boiling H202 for 2 hours, and ultrasonically cleaned with deionized water; while, the samples in the non-oxidation group were not treated with any materials. Alloy leaching liquor was prepared with RPMI-1640 culture media according to the ratio between surface area and volume of culture solution （3 cm2/mL）. The leaching liquor was maintained in incubator at 37 ~C for 96 hours, and then degerming was performed using microporous membrane. 6.4% phenol was added, which was considered as the positive control group, and 1640 culture media was considered as the blank control group. MAIN OUTCOME MEASURES： Growth of L-929 cells was observed under inverted phase contrast microscope; absorbance of cells cultured for 24 and 48 hours was detected using MTT test; cytotexicity of NiTi-SMA with surface oxidation was evaluated. RESULTS： （1） At 24 hours after culture, cells in the positive control group was abnormal; while, cells in the oxidation, non-oxidation, and blank control groups well adherent-grew. At 48 hours after culture, cytotoxicity was detected out in the positive control group; cells in the oxidation, non-oxidation, and blank control groups were normal and grew

关 键 词：镍钛形状记忆合金 表面氧化 细胞毒性 

分 类 号：R318[医药卫生—生物医学工程]