纳米陶瓷人工骨复合血管内皮生长因子修复完全性骨缺损  

Repairing complete bone defect using nano-ceramic artificial bone combined with vascular endothelial growth factor

作　　者：孙勇[1] 肖建德[2] 

机构地区：[1]河南省洛阳正骨医院手外康复中心,河南省洛阳市471002 [2]深圳市第二人民医院骨科,广东省深圳市518035

出　　处：《中国组织工程研究与临床康复》2009年第34期6659-6662,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省卫生厅科技项目资助(2003704)~~

摘　　要：背景:血管内皮生长因子和骨形态发生蛋白是目前公认的对骨生成具有明显促进作用的细胞因子,分别与纳米陶瓷人工骨支架复合,希望可以制造出更好的骨修复材料。目的:比较复合血管内皮生长因子和复合骨形成蛋白的纳米陶瓷人工骨修复骨缺损能力。设计、时间及地点:随机对照动物实验,于2006-08/2007-11在深圳市第二人民医院中心实验室和深圳市药品检验所动物实验室完成。材料:复合重组鼠血管内皮生长因子人工骨中含血管内皮生长因子约300ng/cm3,能有效缓释约10d。复合重组鼠骨形成蛋白人工骨中含骨形成蛋白约80ng/cm3,能有效缓释约12d。方法:将60只新西兰大白兔随机分为2组,在右前下肢中上段用钢锯切除一段2.0cm带骨膜的桡骨后,分别植入复合血管内皮生长因子的纳米陶瓷人工骨(复合血管内皮生长因子组)和复合骨形成蛋白的纳米陶瓷人工骨(复合骨形成蛋白组)。主要观察指标:在术后不同时期行组织切片并染色计算新生骨量及免疫组化血管染色测局部血管的数量。结果:在术后8周前,成骨体积、血管数均是复合血管内皮生长因子组>复合骨形成蛋白组,组织学切片发现在血管长入、骨生成、骨塑型等方面复合血管内皮生长因子组均略早于复合骨形成蛋白组。结论:血管内皮生长因子能明显促进纳米陶瓷人工骨的早期血管化和成骨,复合血管内皮生长因子的纳米陶瓷人工骨骨修复能力与复合骨形成蛋白的纳米陶瓷人工骨相当甚至略强。BACKGROUND： Vascular endothelial growth factor （VEGF） and bone morphogenetic protein （BMP） can obvious promote bone formation. So this paper combined nano-ceramic artificial bone with VEGF or BMP to prepare bone repair materials. OBJECTIVE： To compare the ability of repairing bone defect using Nano-ceramic artificial bone combined with VEGF or BMP. DESIGN, TIME AND SETTING： A randomized control experiment was performed at the central laboratory of Shenzhen Second People＇s Hospital and experimental animal center of Shenzhen Institute for Drug Control from August 2006 to November 2007. MATERIALS： Nano-ceramic artificial bone composite of VEGF containing 300 ng/cm3 VEGF, which can be released within 10 days. Nano-ceramic artificial bone comprised 80 ng/cm3 BMP, which can be released within 12 days. METHODS： A total of 60 New Zealand white rabbits were randomly divided into 2 groups, They were resected a section of bone with periosteum at the middle upside of the radius and implanted into the nano-ceramic artificial bone composite of VEGF or the artificial bone composite of BMP. Specimens were taken and histological and immunohistological analyses were carried out concerning area and density of regenerated and bone number of Vessels, at the different time after operation. MAIN OUTCOME MEASURES： The bone mineral mass of newly formed bone, as well as quantity of blood vessels. RESULTS： Until the 8th week after operation, the density of newly formed bone and the number of regenerated vessels were significantly higher in the nano-ceramic artificial bone composite of VEGF group, and new vessel formation, bone regeneration and maturation in the nano-ceramic artificial bone composite of VEGF group took place in front of the artificial bone composite of BMP group. CONCLUSION： VEGF can promote early vascularization and bone formation of nano-ceramic artificial bone; and the bone repairing capacity is equivalent to, even slightly greater, than the nano-ceramic artificial bone combined with BMP.

关 键 词：纳米陶瓷人工骨 血管内皮生长因子 复合人工骨 细胞因子 

分 类 号：R318[医药卫生—生物医学工程]