噬菌体多肽对IgG抗体吸附的研究  

Preparation of Phage peptide Immunosorbent material and its ability to clear IgG antibody detection

作　　者：刘庆鑫[1] 崔英春[1] 远航 顾华[1] 苗里宁[1] 

机构地区：[1]吉林大学第二医院肾内科,吉林长春130021

出　　处：《中国实验诊断学》2009年第8期1012-1015,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅医学专项基金资助项目(20050404-4)

摘　　要：目的本研究应用噬菌体多肽展示技术,采用人血清IgG抗体作为目标受体,通过筛选获得IgG抗体高度亲和的重组噬菌体,并将这些重组噬菌体与固相支持物偶联,制备免疫吸附材料,检测其吸附IgG抗体的能力。方法筛选噬菌体多肽库,获得与IgG抗体Fc段高度亲和的重组噬菌体并扩增。与活化的琼脂糖颗粒结合,制成能特异结合人血清IgG的免疫吸附材料。采用体外静态吸附法测定吸附血清中IgG能力。结果当反应在30℃下进行2h左右时载体活化率最高。吸附剂对血清中IgG抗体的吸附量随血清比例的增加而增大。当二者混合比达1∶4时,吸附基本饱和,。吸附剂对IgG的清除率随吸附时间的延长而逐渐增加,2h后基本达吸附平衡,在37℃吸附温度,2h吸附时间下,吸附剂对IgG血清中IgG的吸附效率可达49%。IgA、IgM吸附效率分别为34%、49%。结论所制备的噬菌体免疫吸附材料对血清中IgG的具有很强的吸附能力。Objective In this study, peptide phage display technology, using human serum IgG antibody receptor as a target by screening access to a high degree of affinity IgG antibody phage restructuring and reorganization of these phage with the support of the solid-phase-coupling, preparation materials immunosorbent, The detection of IgG antibody absorption. Methods Screening of peptide phage library, and IgG antibody was a high degree of affinity Fc paragraph of the reorganization and expansion of phage. Agarose with the activation of the particles,made of specific binding to human serum IgG ELISA of the material. Using static in vitro assay determination of serum IgG absorption capacity. Results When the reaction in the next 30 ℃ for 2 h when the carrier about the highest rate of activation. Adsorbent of serum IgG antibodies in the serum adsorption with the increase in the ratio increases. When the two mixed up than 1 ： ：4,the basic adsorption saturated. Adsorbent for the removal of igG. with the.absorptian rate of time and gradually increase, 2 h after the adsorption reached a basic balance in the adsorption temperatm＇e of 37℃ ,2 hours adsorption time, the adsorbent of IgG in serum IgG of the efficiency of absorption of up to 49 %. IgA, IgM absorption efficiency of 34%, 49 %. Conclusion The preparation of materials for the phage EUSA in serum IgG hatred with the absorption efficiency.

关 键 词：免疫吸附材料 噬菌体IgG抗体 

分 类 号：R457.2[医药卫生—治疗学]