绿色荧光蛋白基因转染人骨髓间充质干细胞体外成脂肪诱导分化的研究  

Study on induced differentiation adipocyte of human bone marrow mesenchymal stem cells containing EGFP

作　　者：陈晓[1] 宋艳秋[1] 李薇[1] 朱晶[1] 王冠军[1] 

机构地区：[1]吉林大学第一医院肿瘤中心,吉林长春130021

出　　处：《中国实验诊断学》2009年第8期1019-1021,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的以逆病毒为载体将绿色荧光蛋白基因(EGFP)转染人骨髓间充质干细胞(hMSC),研究对其成脂肪诱导分化的影响。方法以逆病毒为载体将EGFP基因转染hMSC,流式细胞仪检测转染后hMSC的表面标记,并对转染EGFP的hMSC进行成脂肪诱导分化培养。结果转EGFP基因的hMSC/EGFP细胞与未转基因的hMSC在细胞形态及生长特点上无差异。转基因的hMSC细胞仍高表达CD29、CD44、CD105抗原,传代培养后可诱导形成脂肪细胞。结论转染EGFP基因对hMSC在体外成脂肪诱导能力无明显影响,EGF可以作为研究hMSC多向分化潜能机制的一个有效示踪工具。Objective To explore the influence of induced differentiation adipocyte of transfection of human bone marrow mesenchymal stem cells （hMSC） with retroviral vector containing enhanced green fluorescent protein （EGFP）. Methods In vitro expanded hMSC transfected with recombinant relroviral vetor containing EGFP, detected its surface marker and differentiation adipocyte. Results There were no significant difference in morphology, surface marker between the hMSC/EGFP and non-transfected cells, and differentiation potential to adipocyte. Conclusion No influence on differentiation potential to adipocyte of hMSC containing EGFP, The EGFP is a reliaable tracer for study in hMSC muhidifferetiation potential.

关 键 词：绿色荧光蛋白 人骨髓间充质干细胞 诱导分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]