镧对软腐欧文氏菌生长及其胞外酶活性的影响  

Effect of Lanthanum on Growth and Extracellular Enzyme Activity of Erwinia Chrysanthemi in vitro

作　　者：刘庆都[1] 章健[2] 承河元[2] 

机构地区：[1]中国科学技术大学生物系 [2]安徽农业大学植物保护系

出　　处：《中国稀土学报》1998年第3期262-266,共5页Journal of the Chinese Society of Rare Earths

摘　　要：研究了镧对软腐细菌（Ｅｒｗｉｎｉａｃｈｒｙｓａｎｔｈｅｍｉ，Ｅｃｈ）生长及胞外酶活性的影响。结果表明，Ｌａ３＋浓度为５０、１００、１５０、２００、２５０、３００和３５０ｍｇ／Ｌ时，固体培养中对Ｅｃｈ生长均呈抑制作用，且随浓度提高，抑制作用加强，表现为菌落出现时间比对照延长，７ｄ后的菌落直径比对照减小。液体培养中，浓度＜２００ｍｇ／Ｌ，Ｌａ在２４ｈ内对Ｅｃｈ生长有轻微刺激作用；但随着浓度提高和培养时间的延长，Ｅｃｈ生长明显受到抑制。当Ｌａ３＋浓度＞３５０ｍｇ／Ｌ，Ｅｃｈ生长基本处于停止状态；浓度为２００ｍｇ／Ｌ，对Ｅｃｈ产生胞外酶能力有一定影响，其中对纤维素酶活性增强最明显，其次是蛋白酶，而对果胶酶活性有减弱作用。经稀土处理的无细胞滤液对马铃薯块茎组织的浸离力降低。The effect of lanthanum on the growth and the extracellular enzyme activity of Erwinia chrysanthei (Ech) was studied. La inhibited the growth of Ech in solid culture at the concentration of 50, 100, 150, 200, 250, 300 and 350 mg/L, and the inhibition effect increased with the rise of the RE concentration. The formation of colony delayed and the diameter of colony decreased. In liquid medium, La stimulated the growth of Ech within 24 h when the concentration was less than 200 mg/L, but significantly inhibited the growth of Ech with the increase of La concentration and culture time. When the concentration was >350 mg/L, the growth of Ech was inhibited completely both in solid and liquid culture. The extracellular enzyme activity of Ech was affected by La at 200 mg/L. The activity of cellulase was promoted more than that of protease, but the activity of pectinase was decreased. The maceration to potato tuber tissue decreased when the cell free filtrate was treated with La.

关 键 词：稀土 镧 软腐欧文氏菌 胞外酶 生长 活性 

分 类 号：S482.2[农业科学—农药学]