大鼠鲍氏不动杆菌性脓毒症血清中肿瘤坏死因子α与白细胞介素-18的变化  

Change in TNF-α and IL-18 Levels in Serum of Rats with Acinetobacter baumannii Sepsis

作　　者：朱晓娜[1] 王邦松[1] 李庆兴[1] 陈迎晓[1] 张慧芳[1] 

机构地区：[1]温州医学院附属第一医院感染内科,浙江温州325000

出　　处：《中华医院感染学杂志》2009年第18期2379-2381,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨肿瘤坏死因子α(TNF-α)和白细胞介素(IL-18)在鲍氏不动杆菌性脓毒症中的变化及意义。方法建立鲍氏不动杆菌大鼠脓毒症模型,按不同时间段分成5个组及1个正常对照组,用ELISA方法监测血清中TNF-α和IL-18的浓度变化。结果TNF-α脓毒症组明显高于正常对照组,差异有统计学意义(P<0.05),各脓毒症组间差异无统计学意义;而IL-18随着时间变化逐渐升高,16h达高峰,明显高于其余各组(P<0.05),之后逐渐下降,甚至低于正常对照组(P<0.05)。结论TNF-α和IL-18在鲍氏不动杆菌性脓毒症中随着不同时间存在一定变化,血清水平的测定将有助于早期诊断。OBJECTIVE To study the change and significance of TNF-α and IL-18 in the Acinetobacter baumannii sepsis. METHODS Sixty male SD rats were divided into 6 groups. The first group was normal control group. The second to sixth groups were sepsis groups which were killed at 4h, 16h, 24h, 48h, 72h after injecting A. baurnan- nii through intraperitoneal injection to make sepsis model. The level of TNF-α and IL-18 in the serum of rats was measured by enzyme linked immunosorbent assay （ELISA）. RESULTS The level of TNF-α in the serum increased markedly in the sepsis groups （P〈0.05）. However, the level of IL-18 in the serum reached the peak at 16h （P〈 0.05）, then decreased gradually. CONCLUSIONS The level of TNF-α and IL-18 in the serum of rats which had A. baumannii sepsis changes at different time. Therefore the serum level of TNF-α and IL-18 may be helpful for earlier diagnosis of A. baurnannii sepsis.

关 键 词：鲍氏不动杆菌 脓毒症 肿瘤坏死因子 白细胞介素-18 

分 类 号：R378[医药卫生—病原生物学]