郑州地区大肠埃希菌中CTX-M型超广谱β-内酰胺酶的基因型分布  

Genotype Distribution of CTX-M type Extended-spectrum β-Lactamases in ESBLs-producing Escherichia coli in Zhengzhou

作　　者：张志坚[1] 郭小兵[2] 阎志勇[3] 张钦宪[3] 

机构地区：[1]郑州大学第三附属医院检验科,河南郑州450052 [2]郑州大学第一附属医院检验科,河南郑州450052 [3]郑州大学基础医学院,河南郑州450052

出　　处：《中华医院感染学杂志》2009年第18期2382-2384,共3页Chinese Journal of Nosocomiology

基　　金：河南省医学科技攻关项目(200703071)

摘　　要：目的了解郑州地区CTX-M型ESBLs在大肠埃希菌中的基因型分布。方法收集郑州大学第一、三附属医院2006-2007年临床分离产ESBLs不重复大肠埃希菌138株,小量提取菌株质粒,依据CTX-M型ESBLs基因同源性分析结果,设计4对简并引物,采用各对引物对所提质粒进行PCR扩增,阳性扩增产物纯化后直接测序,测序结果与blaCTX-M比对后明确流行型别。结果138株产ESBLs大肠埃希菌中共检出CTX-M型ESBLs80株,具有单一CTX-M型别者53株,同时具有两种CTX-M型别者25株,3种CTX-M型别者2株;CTX-M型ESBLs中,CTX-M-1(43株)、CTX-M-14(56株)、CTX-M-25(7株)及CTX-M-38(5株)。结论郑州地区大肠埃希菌CTX-M型ESBLs以CTX-M14型为主,且同一菌株可有多种CTX-M酶型。OBJECTIVE To study the genotype distribution of CTX-M type ESBLs in ESBLs-producing Escherichia coli. METHODS All 138 strains of E. coli were collected from the First and Third Affiliated Hospitals, Zhengzhou University from 2006 to 2007. The CTX-M type ESBLs genes were amplified by PCR, the products were sequenced after purification. RESULTS Eighty-two of 138 ESBLs-producing E. coli isolates were classified with CTX-M type-ESBLs genes,and there were 53,27 and 2 strains contained only one type, two types and three types of CTX-M ESBLs, respectively. Among CTX-M type-ESBLs genes,they were identified with CTX-M-1（43 strains）, CTX-M-14 （56 strains）, CTX-M-25 （7 strains） and CTX-M-38 （5 strains）. CONCLUSIONS The most popular type of CTX-M ESBLs in E. coli is CTX-M14 in Zhengzhou,and two or three kinds of genotypes can exist in 1 strain.

关 键 词：CTX-M 超广谱Β-内酰胺酶 PCR基因型 

分 类 号：R378.21[医药卫生—病原生物学]