金黄杆菌属检出aac(6′)-Ⅱ和ant(2″)-Ⅰ耐药基因研究  

Detection of Two Types of Aminoglycoside-modifying Enzymes Genes in Chryseobacterium spp

作　　者：明德松[1] 张志珊[1] 庄建良[1] 苏智军[1] 谢尊金[1] 

机构地区：[1]福建医科大学附属泉州第一医院检验科,福建泉州362000

出　　处：《中华医院感染学杂志》2009年第18期2387-2389,共3页Chinese Journal of Nosocomiology

摘　　要：目的研究金黄杆菌属临床分离株6种氨基糖苷类耐药机制。方法应用法国生物梅里埃公司的API鉴定条/PSE5.0药敏条进行细菌鉴定和药敏试验,应用PCR法检测两株金黄杆菌属临床分离株6种氨基糖苷类修饰酶基因(AMEs)耐药基因,并对PCR阳性产物测序和同源性分析。结果在两株临床分离菌株均检出2种AMEs基因(aac(6′)-Ⅱ、ant(2″)-Ⅰ),同源性分析证实为2种AMEs基因,GenBank注册号分别为EU252512,EU252513,EU252514,EU252515;其他4种AMEs基因(aac(6′)-Ⅰb、aac(3)-Ⅱ、ant(3″)-Ⅰ、aac(3)-Ⅰ)均阴性。结论金黄杆菌属临床分离株存在AMEs基因,且为两种基因共存。OBJECTIVE To study 6 kinds of aminoglycoside-modifying enzymes （AMEs） genes in Chryseobacterium spp isolates. METHODS The isolates were identified by API20NE Gram-negative identification cards, and the susceptibility of antimicrobial agents was detected by MIC kits （ bioMerieux ） 6 AMEs genes of 2 strains of Chryseobacterium spp were measured by PCR, and verified by DNA sequencing and sequence analysis. RESULTS In the 2 strains, 2 kinds of resistant genes [aac（6′）-Ⅱ and ant（2″）-Ⅰ ] were positive, and 4 kinds genes of AMEs [aac（6′）-Ⅰ b, aac（3）-Ⅱ , aac（3″）- Ⅰ and aac（3）- Ⅰ ] were negative. The amplicons were purified, sequenced and analyzed withBLAST 2. 0 and found to be identical to aac （6′）-Ⅱ and ant （2″）-Ⅰ. CONCLUSIONS There are AMEs in Chryseobacterium spp isolates. This is the first report on AMEs genes [coexistance of aac（6′）-Ⅱ and ant（2″）-Ⅰ] in Chryseobacterium spp.

关 键 词：金黄杆菌属 氨基糖苷类修饰酶基因 aac(6′)-Ⅱ ant(2″)-Ⅰ 

分 类 号：R378[医药卫生—病原生物学]