人参皂甙预防SAMP8小鼠空间参考记忆减退的作用和机制实验研究  

The Prevention the Memory Loss Through Long-term Ginsenoside Consumption in Aged SAMP8 Mice by Decreasing Oxidative Stress

作　　者：赵海峰[1,2] 李琼[1] 裴新荣[1] 张召锋[1] 王军波[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100191 [2]山西医科大学公共卫生学院营养与食品卫生学教研室．太原030001

出　　处：《科技导报》2009年第17期52-56,共5页Science & Technology Review

基　　金：国家“十一五”科技支撑项目(2006BAD27B08)

摘　　要：为探讨人参皂甙预防SAMP8小鼠空间参考记忆减退的作用和机制,本研究选用SPF级4月龄雄性SAMP8小鼠,将小鼠随机分为快速老化对照组和人参皂甙低、中、高3个剂量干预组,每组17只。人参皂甙3个剂量分别为50,100,200mg/(kg·d),随饮水摄入,共干预7个月。另外,设同月龄的SAMR1小鼠作为正常老化对照组。干预结束后,利用Morris水迷宫实验检测空间记忆参考能力。记忆检测时,加设4月龄SAMP8小鼠作为青年对照组。行为学检测完毕后,测定各组动物血清T-SOD、MDA及GSH-Px含量、小鼠海马神经元超微结构的改变以及海马组织中Aβ1-42的表达。结果显示,人参皂甙100,200mg/kg可以预防老年SAMP8小鼠空间参考记忆的减退,其增加小鼠体内的抗氧化水平、减轻海马神经元的氧化损伤以及减少Aβ1-42在海马组织中的沉积是改善记忆的重要机制。To investigate the preventive effect of ginsenoside on the spatial reference memory impairment in Senescence Accelerated Mouse Prone 8 （SAMP8） and the mechanisms, in the present study, SAMP8 mice aged 4 months were chronically treated with ginsenoside （given in drinking water： in 3 dose groups and for 7 months）. The 3 dose groups were administered with ginsenoside of 50, 100 and 200 mg/kg per day, respectively. Placebo-treated aged mice and young mice （4 months old）were used as controls. In addition, Senescence Accelerated Mouse Resistant 1 （SAMR1） mice were used as ＂normal aging＂ control. The beneficial role of ginsenoside was manifested in the prevention of spatial reference memory loss in aged SAMP8 mice. The optimal dose of ginsenoside is 100 or 200 mg/kg per day In ginsenoside treated groups, the antioxidase level in serum is significantly increased. However, the neurons damage level of the uhrastructure and the Aβ1-42 level markedly decreased in hippocampus. These findings suggest that the increase of antioxidation may be one of mechanisms of the memory loss prevention of ginsenoside in aged SAMP8 mice.

关 键 词：人参皂甙 SAMP8小鼠 空间参考记忆 氧化应激 

分 类 号：R285.5[医药卫生—中药学]