冈村枝管藻叶绿体psbA基因的克隆及序列分析  

Cloning and sequence analysis of psbA gene fragment from the chloroplast of Cladosiphon okamuranus

作　　者：朱清华[1] 张学成[2] 李玉晖[3] 

机构地区：[1]德州学院生物系,山东德州253023 [2]中国海洋大学海洋生命学院,山东青岛266003 [3]合肥学院生物与环境工程系,安徽合肥230022

出　　处：《海洋科学》2009年第9期20-24,共5页Marine Sciences

基　　金：农业部引进国际先进农业科学技术(948)项目(2003-Z104)

摘　　要：psbA基因编码光合系统II反应中心的D1蛋白,是叶绿体基因组中一个重要的光调控基因。根据网管藻(Dictyosiphon foeniculaceus)、髋藻(Myelophycus simplex)、粗粒藻(Asperococcus fistulosus)、索藻(Chor-daria flagelliformis)、点叶藻(Punctaria latifolia)、水云(Ectocarpus siliculosus)、铁钉菜(Ishige okamu-rae)、绳藻(Colpomeniasinuosa)、网胰藻(Hydroclathrus clathratus)、幅叶藻(Petalonia fascia)等10种藻类的psbA基因高度保守序列,设计引物,利用PCR方法从冈村枝管藻(Cladosiphon okamuranus)基因组DNA中扩增出约750 bp的片段,将该片段连接到pMD18-T载体上进行序列测定。结果表明:片段长度为737bp,推导的245个氨基酸序列与网管藻、髋藻、粗粒藻、索藻、点叶藻、水云、铁钉菜、绳藻、网胰藻、幅叶藻的D1蛋白相对应的氨基酸序列的同源性均高于96%。该基因序列已被GenBank收录,登录号为EU332142。According to conserved motif of the homologous nucleotide sequence of psbA of ten kinds of algae, a pair of primers were designed and about 750 bp fragment was amplified from the genome of Cladosiphon okarnuranus. The resulting PCR products were inserted into pMD18-T vector and sequenced. The results showed that the nucleotide sequence was 737 bp and the sequence shared high homology with identity above 96% to the psbA of Dictyosiphon foeniculaceus, Myelophycus simplex, Asperococcus fistulosus, Chordaria flagelli f orrnis , Punctaria lati f olia , Ectocarpus siliculosus , Ishige oharnurae , Col pornenia sinuosa, Hydroclathrus clathratus and Petalonia fascia. The psbA gene was accepted by GenBank （Accession number. EU332142）. 245 amino acids were deduced from 737 bp nucleotide. Analyzing the codon usage of psbA,we found that there were problems of degenerate and preference of codon usage.

关 键 词：冈村枝管藻(Cladosiphon okamuranus) PCR扩增 PSBA基因 序列分析 

分 类 号：Q178.53[生物学—水生生物学] Q7[生物学—普通生物学]