黄鳍金枪鱼巨噬细胞移动抑制因子(MIF)的克隆与序列分析  

Molecular cloning and sequence analysis of macrophage migration inhibitory factor (MIF) gene from Thunnus albacares

作　　者：毛勇[1,2] 王军[1] 张之文[1] 王定[1] 苏永全[1] 

机构地区：[1]厦门大学海洋环境学院,福建厦门361005 [2]广东海洋大学水产学院,广东湛江524025

出　　处：《海洋科学》2009年第9期29-34,共6页Marine Sciences

基　　金：福建省科技重大专项(2004NZ03)

摘　　要：采用同源克隆的方法、利用RACE技术从黄鳍金枪鱼(Thunnus albacares)肝脏中克隆了其MIF(TaMIF)的cDNA序列。TaMIF的cDNA全长706 bp含一个345 bp的开放阅读框,编码一个长115氨基酸的蛋白质。信号肽预测分析表明TaMIF没有信号肽。序列分析与进化分析表明,黄鳍金枪鱼与近海养殖鱼类MIF的氨基酸序列高度相似、进化地位相近,预示着深海鱼类TaMIF与近海养殖鱼类具有相似的生物学功能。研究结果是对深海鱼类MIF基因信息资料的重要补充。The cDNA sequence of an MIF homologue （TaMIF） was cloned from the liver of Thunnus albacares by a homologous cloning strategy and RACE approach. The fulldength cDNA of TaMIF was 706 bases long and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. Predication of the signal peptide indicated that TaMIF lacks a signal peptide. Sequence analysis revealed a high structural similarity and phylogenetic analysis indicated a close relationship between TaMIF and other fish MIF, implying that TaMIF maybe have a simliar function to offshore cultured fish MIFs. The result is the complement of the gene information to open-sea fish MIF gene.

关 键 词：黄鳍金枪鱼(Thunnus albacares) 巨噬细胞移动抑制因子(MIF) 分子克隆 序列分析 

分 类 号：Q173[生物学—水生生物学]