细粒棘球蚴细胞系13G-5培育  

Cultivation of an Echinococcus granulosus Larvae Cell Line

作　　者：陆家海[1] 程维兴[1] 郭中敏[1] 孔长青 冯德元[1] 李德昌[1] 郭固[1] 

机构地区：[1]兰州军区乌鲁木齐总医院

出　　处：《中国兽医学报》1998年第5期479-482,共4页Chinese Journal of Veterinary Science

基　　金：军队医药卫生青年基金

摘　　要：从１８例棘球蚴病人的包囊分离棘球蚴细胞，经３６批次体外培养获得１株细粒棘球蚴细胞系（１３Ｇ－５细胞系），已培养１４０ｄ，传２１代，并继续扩大增殖培养。测定了该细胞系第１３代细胞生长曲线和集落形成率（４％）。１３Ｇ－５细胞系５代以前为多形性细胞，后逐渐以成纤维型细胞占优势。探讨了棘球蚴细胞培养的制约因素。The Echinococcus larvae cells were obtained from the echinococcal cyst excised surgically from patients with liver echinococcosis. Primary cell cultures were set up 36 times, but only the cell line of E granulosus (13G 5) from a 7 year girl was subcultured successfully. The growth curve of the 13G 5 cell line and its plating efficiency (PE) at passage 13 were determined. Though cells of 13G 5 cell line survived for up to 140 days and subcultured 21 passage up to date they had still been multiplying well in vitro. The cultivated cells before passage 5 varied in size and shape, thereafter the fibroblast like cells in which came to be dominant. Some restrictive factors involved in the cultivation of echinococcus larvae cells were discussed as well.

关 键 词：棘球蚴病 细粒棘球绦虫 体外培养 13G-5细胞系 

分 类 号：R383.33[医药卫生—医学寄生虫学]