霉菌Y3菌丝球培养条件的优化  

Optimization of culturing conditions for mucedine Y3 pellet

作　　者：马放[1,2] 张斯[1,2] 山丹[1,2] 高敏[1,2] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090 [2]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《哈尔滨工业大学学报》2009年第8期38-41,共4页Journal of Harbin Institute of Technology

基　　金：国家重点基础研究发展计划资助项目(973-2004CB185050);黑龙江省杰出青年基金资助项目(JC200601)

摘　　要：为找到一种菌丝球培养的快捷、经济的系统方法,以用于工程上菌丝球的批量生产,通过对环境因子的逐一筛选,进而对菌丝球的培养条件进行优化.结果表明:在孢子龄20～26d、接种量104个/L、培养温度37℃、培养基初始pH为5、摇床转速160r/min、CaCl2质量分数0.02%、无表面活性剂(tween80)的条件下,形成的菌丝球综合性能最佳,对中性黄的脱色效果最好,达99%以上.同时,对菌丝碎片作为菌丝球传代手段的可行性研究证明,菌丝球在1800r/min粉碎机中粉碎30s,所形成的菌丝碎片碎度最佳.In order to obtain a systematic method for rapid and economic mass culturing of mycelium pellets in practical engineering, preliminary experiments were carried out to screen each environmental factor, then to optimize the culturing conditions. It was found that when the age of spore was 20 -26 d, the inoculums concentration was 104/L, the eulturing temperature was 37 %, the initial culturing pH was 5. 0, the speed of shaking table was 160 r/min, the percentage composition of CaC12 was 0. 02%, and the tween80 was absent in the medium, mycelium pellets showed high activity and good color removing efficiency of 99% for Neutral Yellow. Experimental result verities that the optimal ground pellets can be obtained by grinding at 1800 r/min for 30 s.

关 键 词：菌丝球 传代方法 脱色 

分 类 号：X703[环境科学与工程—环境工程]