葡萄籽提取物原花青素对人膀胱癌BIU87细胞增殖和凋亡的影响  

Effects of grape seed procyanidin extract on proliferation and apoptosis of human bladder cancer cell line BIU87

作　　者：刘洁[1] 庄乾元[1] 张维怡[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉市430030 [2]华中科技大学同济医学院附属同济医院中西医结合科,武汉市430030

出　　处：《实用医学杂志》2009年第18期3000-3001,共2页The Journal of Practical Medicine

摘　　要：目的:探讨葡萄籽提取物原花青素(GSPE)对人膀胱癌细胞株BIU87细胞增殖和凋亡的影响。方法:BIU87细胞与50、100和200μg/mL的GSPE共同孵育24h后,采用噻唑蓝(MTT)法检测细胞增殖抑制率,流式细胞术分析细胞凋亡情况。结果:不同浓度(50、100和200μg/mL)GSPE对BIU87细胞的增殖抑制率分别为(13.0±1.5)%、(31.8±2.1)%和(48.6±1.8)%;凋亡率分别为(8.7±0.7)%、(28.2±1.6)%和(48.5±0.7)%;细胞增殖抑制率和凋亡率均随GSPE浓度的升高而增高(P<0.01)。结论:GSPE在体外可呈浓度依赖性抑制膀胱癌BIU87细胞增殖并促进其凋亡。Objective To investigate the effects of grape seed procyanidin extract （GSPE） on proliferation and apoptosis of human bladder cancer cell line BIU-87. Methods BIU-87 cells were cultured with different concentrations of GSPE （50, 100, or 200 μg/mL） for 24 hours, in vitro inhibition of cell proliferation was measured by MTf assay, and apoptosis was detected by flow cytometry. Results The inhibition rate was （13.0 ± 1.5）%, （31.8 ± 2.1）%, and （48.6 ± 1.8）%, respectively, while the apoptotie rate was （8.7 ± 0.7）%, （28.2± 1.6）%, and （48.5 ± 0.7）%. Both the inhibition rate and the apoptotic rate elevated with the increasing GSPE concentration （P 〈 0.01 ）. Conclusion GSPE inhibits the growth of BIU-87 cells and induces apoptosis in vitro in a concentration-dependent manner.

关 键 词：膀胱肿瘤 葡萄籽提取物原花青素 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]