基于分子印迹识别的化学发光微流控传感器芯片测定双嘧达莫  

Chemiluminescence Determination of Dipyridamole with Microfluidic Sensor Chip Based on Molecular Imprinted Recognition

作　　者：何德勇[1] 章竹君[2] 何树华[3] 

机构地区：[1]井冈山学院化学化工系,吉安343009 [2]西南大学化学化工学院,重庆400715 [3]长江师范学院化学系,重庆408100

出　　处：《理化检验（化学分册）》2009年第9期1025-1028,1031,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(30470886);江西省教育厅科技项目(GJJ08413)

摘　　要：试验中,以双嘧达莫为模板分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,氯仿为溶剂,合成了双嘧达莫分子印迹聚合物。将此聚合物填充在长8 mm,宽1 mm,深0.5 mm的微流控芯片检测池中作为分子识别物质,设计了一种新型的化学发光微流控传感器芯片测定双嘧达莫。双嘧达莫被此聚合物在线吸附并识别,被吸附的双嘧达莫与鲁米诺和铁氰化钾混合溶液反应并导致其化学发光强度增大。该传感器对双嘧达莫响应范围为1.0～20μg·L^(-1),检出限(3σ)为0.5μg·L^(-1),对10μg·L^(-1)双嘧达莫连续平行测定7次,其相对标准偏差为4.6%。A new chemiluminescence microfluidic sensor chip based on molecular-imprinted recognition, used for determination of dipyridamole was designed and fabricated. The molecular imprinted polymer was synthesized by using dipyridamol as template molecule, a-methyl propenoic acid as functional monomer and ethylene glycol di-σ-methyl propenoate as cross-linking agent and with chloroform as solvent. The polymer obtained was filled into the detection cell of the microfluidic sensor chip, with its dimension of 8 mm（length）×1 mm（width）×0. 5 ram（depth）, acting as recognition element. Dipyridarnole was on-line adsorbed and recognized by the molecular-imprinted polymer of the sensor chip, and the adsorbed dipyridamole was reacted with a mixed solution of luminol and potassium ferricyanide, resulting an enhancement of chemiluminence （CL） signal. The magnitude of increase of CL intensity kept a linear relationship with mass concentration of dipyridarnole in the range of 1.0-20μg·L^-1. Detection limit （3σ） of the method was found to be 0. 5μg·L^-1. Test for precision was made at the concentration level of 10μg·L^-1 of dipyridamole, and value of RSD （n=7） found was 4.6%.

关 键 词：微流控传感器芯片 分子印迹识别 化学发光 双嘧达莫 

分 类 号：O657[理学—分析化学]