高效液相色谱法测定均三甲苯氧化产物及其中间产物  

HPLC Determination of Main Oxidation Product of Mesitylene and Its Intermediate Products

作　　者：邱方利[1] 李呈宏[1] 梁华定[1] 

机构地区：[1]台州学院医药化工系,临海317000

出　　处：《理化检验（化学分册）》2009年第9期1048-1050,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：浙江省自然科学基金资助项目(Y405377)

摘　　要：采用高效液相色谱法同时测定均三甲苯及其氧化生成的均苯三甲酸、3,5-二甲基苯甲酸和5-甲基-1,3苯二甲酸的含量。对色谱分离测定条件做了试验并予以优化。以SHIMADZUC_(18)色谱柱(150 mm×4.6 mm,5μm)为分离柱,以甲醇、水和pH 5.0乙酸盐缓冲溶液以体积比79比20比1的混合溶液为流动相,在检测波长为225 nm处进行测定。均三甲苯和3个氧化产物质量浓度在一定范围内与其峰面积呈线性关系,标准加入回收率分别在99.5%～100.4%,99.7%～100.8%,99.6%～100.8%和99.3%～101.0%之间,相对标准偏差(n=6)均小于2.0%。HPLC was applied to the determinations of the main oxidation and intermediate products of mesitylene, including trimesic acid, mesitylenic acid and 5-methyl-1, 3-benzenedicarboxylic acid. The conditions for separation and determination by HPLC were tested and optimized. The SHIMADZU 1218 column （150 mm×4. 6 mm, 5 μm） was used as stationary phase, and a mixed solution of methanol, water and pH 5. 0 acetate buffer solution （mixed in the a ratio of 79 to 20 to 1） was used as mobile phase. UV-detection at 225 nm was adopted in the determination. Linear relationships between the values of peak area and mass concentration of mesitylene, trimesic acid, mesitylenic acid and 5-methyl-1, 3-benzenedicarboxylic acid in definite ranges were obtained. The values of recovery found by standard addition method were in ranges of 99. 5%- 100. 4%, 99. 7%- 100. 8%, 99. 6%-100. 8% and 99. 3%--101.0% respectively. The values of RSD＇s （n=6） were less than 2. 0%.

关 键 词：高效液相色谱法 均三甲苯 均苯三甲酸 3 5-二甲基苯甲酸 5-甲基-1 3苯二甲酸 

分 类 号：O657.7[理学—分析化学]