维生素E合成途径关键酶基因的克隆及转基因植株构建  

Cloning of Key Enzyme Genes Involved in Vitamin E Biosynthesis and Acquirement of Transgenic Plants

作　　者：叶从勇[1] 刘南波[1] 郑穗平[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《现代食品科技》2009年第9期1020-1023,共4页Modern Food Science and Technology

基　　金：广东省农业科技攻关项目;粤科计字[2006]621号

摘　　要：维生素E是一种脂溶性抗氧化剂,能够清除生物膜上自由氧基及脂过氧原子团,多应用于医药、食品和化妆品等方面。而尾穗苋为一年生草本植物,富含维生素和蛋白质,是一种良好的牲畜青饲料。因此通过代谢工程方法对植物进行品质改良,提高植物体内维生素E含量以提高其经济价值具十分重要意义。本研究从拟南芥中克隆得到维生素E合成途径中的尿黑酸植基转移酶基因、γ-生育酚转移酶基因并连接到植物表达载体pBI121,以尾穗苋子叶节作为转化受体,通过农杆菌介导的方法,将γ-生育酚转移酶基因成功转入尾穗苋。Tocopherols （vitamin E） are lipid-soluble antioxidants possessing abilities to scavenge reactive oxygen species and lipid peroxy radicals in biomembranes, which are mainly used in medicine, food, cosmetic and so on. Amaranthus caudatus Linn., an annual herb containing high quality of vitamins and proteins, can be used as silage for animals. So evaluation of the levels of vitamin E in plant through metabolic engineering was important for the improvement of the nutritional quality and economic value of the crop. In this research, genes of homogentisate phytyltransferase （HPT/vte2） and γ-tocopherol methyltransferase （γ-TMT/vte4） involved in tocopherol synthesis in Arabidopsis were cloned and constructed into plant expression vector pBI121. By using Agrobacterium Tumefaciens LBA4404 as donor strains and cotyledonary nodes as recipient material, γ-tocopherol methyltransferase（γ-TMT/vte4） was introduced into Amaranthus caudatus successfully.

关 键 词：尾穗苋 维生素E 子叶节 γ-生育酚转移酶 

分 类 号：Q943.2[生物学—植物学]